Chinese Journal OF Rice Science >
Effects of Nitric Oxide on Drought Stress-induced Physiological Characteristics in Leaves of Nipponbare (Oryza sativa L)
Received date: 2014-07-14
Revised date: 2014-10-20
Online published: 2015-01-10
To reveal the mechanisms that nitric oxide (NO) enhances drought tolerance in rice, we studied the effects of NO on leaf photosynthetic rate, relative water content and antioxidant system of rice under drought stress. After foliar spray of 100 μmol/L sodium nitroprusside (SNP, a NO donor) and/or 100 μmol/L cPTIO (a NO scavengers), japonica nipponbare (Oryza sativa L) was subjected to drought stress during tillering stage in a plastic tank. Rice leaves were sampled for further physiological analysis at the sixth day after treatment. Results showed that foliar spray of 100 μmol/L SNP enhanced rice tolerance to drought stress, which showed higher photosynthetic rate and relative water content, but lower malonaldehyde (MDA) and hydrogen peroxide (H2O2) contents in leaves when compared with the control, foliar spraying with deionized water. By contrast, the cPTIO treatment significantly reduced leaf photosynthetic rate and relative water content, and significantly increased leaf MDA and H2O2 contents. Further study indicated that foliar application of SNP significantly increased superoxide dismutase (SOD) and catalase (CAT) activities, but observably suppressed ascorbic acid (AsA) content and total glutathione (GSH+GSSH) contents under drought stress. However, it had no significant influence on peroxidase (POD) activity, ascorbate peroxidase (APX) activity and there were no significant difference among the four treatments. It was concluded that nitric oxide might increase the activity of SOD and CAT, resulting in improved drought tolerance in rice.
Key words： rice; drought stress; nitric oxide; antioxidant system
Yong-jie YANG, Xue-qin YANG, Cai-xia ZHANG, Guan-fu FU, Ting-ting CHEN, Long-xing TAO . Effects of Nitric Oxide on Drought Stress-induced Physiological Characteristics in Leaves of Nipponbare (Oryza sativa L)[J]. Chinese Journal OF Rice Science, 2015 , 29(1) : 65 -72 . DOI: 10.3969/j.issn.1001-7216.2015.01.008
| [1] | 杨永杰, 张彩霞, 宋建, 等. 花期干旱胁迫对籼稻近等基因系水分和光合生理的影响. 中国农业科学, 2013, 46(7): 1481-1491. |
| [2] | Hu H H, Dai M Q, Yao J L,et al.Over expressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice.PNAS, 2006, 103(35): 12987-12992. |
| [3] | Ning J, Li X H, Hicks L M, et al.A raf-like MAPKKK gene DSM1 mediates drought resistance through reactive oxygen species scavenging in rice.Plant Physiol, 2010, 152(2): 876-890. |
| [4] | 陶龙兴, 谈惠娟, 王熹. 灌溉稻田“麦作式”水稻旱种技术. 中国稻米, 2005, 12(3): 24-25. |
| [5] | Shinozaki K, Yamaguchi-Shinozakiy K, Sekiz M.Regulatory network of gene expression in the drought and cold stress responses.Curr Opin Plant Biol, 2003, 6(5):410-417. |
| [6] | 艾林, 李召虎, 李建民, 等. 植物生长物质冠菌素诱导旱稻、水稻幼苗抗旱性的效应及生理机制. 中国水稻科学, 2008, 22(4): 443-446. |
| [7] | Luna C M, Pastori G M, Driscoll S,et al.Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat.J Exp Bot, 2005, 56(411): 417-423. |
| [8] | Xiong J, Fu G F, Yang Y J, et al.Drought-induced proline accumulation is uninvolved with increased nitric oxide, which alleviates drought stress by decreasing transpiration in rice.J Plant Res, 2012, 125(1): 155-164. |
| [9] | Garcı'a-Mata C, Lamattina L. Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress.Plant Physiol, 2001, 126(3): 1196-1204. |
| [10] | Fan Q J, Liu J H.Nitric oxide is involved in dehydration/drought tolerance in Poncirus trifoliata seedlings through regulation of antioxidant systems and stomatal response.Plant Cell Rep, 2012, 31(1): 145-154. |
| [11] | 相昆, 李宪利, 张美勇, 等. 外源一氧化氮对核桃幼苗抗旱性的影响. 林业科学, 2007, 43(10): 122-126. |
| [12] | 赵立群, 刘玉良, 孙宝腾, 等. 干旱胁迫下一氧化氮对小麦离体根尖离子吸收的影响.作物学报, 2009, 35(3): 530-534. |
| [13] | Tian X, Lei Y.Nitric oxide treatment alleviates drought stress in wheat seedlings.Biol Plant, 2006, 50(4): 775-778. |
| [14] | 赵丽英, 邓西平, 山仑. 活性氧清除系统对干旱胁迫的响应机制. 西北植物学报, 2005, 25: (2): 413-418. |
| [15] | Tan J F, Zhao H J, Hong J P,et al.Effects of exogenous nitric oxide on photosynthesis, antioxidant capacity and proline accumulation in wheat seedlings subjected to osmotic stress.World J Agric Sci, 2008, 4(3): 307-313. |
| [16] | 符冠富, 陶龙兴, 宋健, 等. 花期干旱胁迫对籼稻近等基因系育性的影响. 中国水稻科学, 2011, 25(6): 613-620. |
| [17] | Arasimowicz-Jelonek M, Floryszak-Wieczorek J, Kubis J.Involvement of nitric oxide in water stress-induced responses of cucumber roots.Plant Sci, 2009, 177(6): 682-690. |
| [18] | 霍学敏, 呼天明, 杨培志, 等. 干旱胁迫对苗期紫花苜蓿3种生理指标的影响. 草业科学, 2010, 27(4): 89-92. |
| [19] | Zheng Y H, Jia A J, Ning T Y,et al.Potassium nitrate application alleviates sodium chloride stress in winter wheat cultivars differing in salt tolerance.J Plant Physiol, 2008, 165(4): 1455-1465. |
| [20] | Yang Y J, Cheng L M, Liu Z H.Rapid effect of cadmium on lignin biosynthesis in soybean roots.Plant Sci, 2007, 172(3): 632-639. |
| [21] | Thordal-Christensen H, Zhang Z G, Wei Y D, et al.Subcellular localization of H2O2 in plants, H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction.Plant J, 1997, 11(6): 1187-1194. |
| [22] | 夏弈明. 总谷胱甘肽的测定方法. 营养学报, 1990, 12(1): 18-22. |
| [23] | 贾海红, 韩宝平, 林晶, 等. 间接分光光度法测定抗坏血酸. 化工时刊, 2006, 20(12): 34-36. |
| [24] | Nakano Y, Asada K.Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast.Plant Cell Physiol, 1981, 22(5): 867-880. |
| [25] | Delledonne M, Zeier J, Marocco A, et al.Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response.PNAS, 2001, 98(23): 13454-13459. |
| [26] | Farooq M, Basra S M A, Wahid A,et al. Exogenously applied nitric oxide enhances the drought tolerance in fine grain aromatic rice (Oryza sativa L.).J Agron Crop Sci, 2009, 195(4):254-261. |
| [27] | Naverre D A, Wendehenne D, Dumer J,et al.Nitric oxide modulates the activity of tobacco aconitase.Plant Physiol, 2000, 122(2): 573-582. |
| [28] | 刘建新, 王鑫, 李博萍. 外源一氧化氮供体SNP对NaCl胁迫下黑麦草幼苗叶片抗坏血酸-谷胱甘肽循环的影响. 草业学报, 2010, 19(2): 82-88. |
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