Research Papers

QTL Analysis on New Root Traits after Rice Transplanting

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  • Cultivation and Farming Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086; 

Received date: 2014-01-10

  Revised date: 2014-03-03

  Online published: 2014-11-10

Abstract

To reveal the genetic basis of the new root  traits after transplanting, by using Sasanishiki, Habataki and 85 backcross recombinant inbred lines (BC1F5) and its linkage map including 245 molecular markers QTL controlling new root   traits after transplanting were detected. Ten QTLs controlling average diameter, total root length, average surface area, root length and root number were identified. These QTLs distributed on chromosome 1, 2, 5, 9 and 11. The phenotypic variations (VE) explained by individual QTL ranged from 10.7% to 28.5%. QTL clusters were formed on chromosome 9 and 11. Then four major QTLs were fine mapped by “selective mapping” method. The QTLs (qRL11.1,qASA11 and qRN11.1) for root length, average surface area and root number were located between C477 and G320B (5 461 121-6 686 166 bp) on chromosome 11. It was a novel locus with research and application value. 

Cite this article

JIANG Shukun, ZHANG Fengming*, BAI Liangming, SUN Shichen, WANG Tongtong, DING Guohua, JIANG Hui, ZHANG Xijuan . QTL Analysis on New Root Traits after Rice Transplanting[J]. Chinese Journal OF Rice Science, 2014 , 28(6) : 598 -604 . DOI: 10.3969/j.issn.1001-7216.2014.06.005

References

\[1\]IRRI. Rice Almanac. 3rd end. Los Baos, Philippines: International Rice Research Institute, 2002.

\[2\]卢百关, 秦德荣, 樊继伟, 等. 江苏省直播稻生产现状、趋势及存在问题探讨. 中国稻米, 2009(2): 4547.

\[3\]徐迪新, 徐翔. 中国直播稻、移栽稻的演变及播种技术的发展. 中国稻米, 2006(3): 69.

\[4\]张凤鸣, 金官植, 金学泳. 寒地水稻旱育稀植技术. 黑龙江农业科学, 1988(2): 3235.

\[5\]杨春华. 绿色的希望——记水稻旱育稀植技术. 农村工作通讯, 1996(3): 4243.

\[6\]任万军, 张怀渝, 杨文钰, 等. 水稻移栽后苗期茎叶光合产物运转与分配特性研究. 作物学报, 2007, 33(12): 20672070.

\[7\]解晓东, 王伯伦,王术, 等. 水稻不同育苗移栽方式的比较研究. 沈阳农业大学学报, 2001, 32(5): 328332.

\[8\]王松良, 林文雄. 水稻旱育稀植高产机理和调控技术:I.水稻旱育稀植高产机理研究进展与展望. 福建农业大学学报, 1999, 28 (1): 1217.

\[9\]王松良, 林文雄, 梁义元, 等. 水稻旱育稀植高产机理和调控技术:Ⅱ. 水稻旱育稀植高产的生态学机理. 福建农业大学学报, 1999, 28(2): 135141.

\[10\]Horii H, Nemoto K, Miyamoto N, et al. Quantitative trait loci for adventitious and lateral roots in rice. Plant Breeding,  2006, 125, 198200.

\[11\]Price A H, Steele K A, Moore B J, et al. A combined RFLP and AFLP linkage map of upland rice (Oryza sativa L.) used to identify QTLs for rootpenetration ability. Theor Appl Genet,  2000, 100: 4956.

\[12\]Price A H, Steele K A, Moore B J, et al. Upland rice grown in soilfilled chambers and exposed to contrasting waterdeficit regimes: Ⅱ. Mapping quantitative trait loci for root morphology and distribution. Field Crops Res,  2002, 76: 2543.

\[13\]Courtois B, Shen L, Petalcorin W, et al. Locating QTLs controlling constitutive root traits in the rice population IAC 165 × Co39. Euphytica,  2003, 134: 335345.

\[14\]Uga Y, Okuno K, Yano M. QTLs underlying natural variation in stele and xylem structures of rice root. Breeding Sci,  2008,  58: 714.

\[15\]Topp C N, IyerPascuzzi A S, Anderson J T, et al. 3D phenotyping and quantitative trait locus mapping identify core regions of the rice genome controlling root architecture. Proc Natl Acad Sci USA, 2013, 110(18): 16951704.

\[16\]Li Z, Mu P, Li C,et al. QTL mapping of root traits in a doubled haploid population from a cross between upland and lowland japonica rice in three environments. Theor Appl Genet,  2005, 110(7): 12441252.

\[17\]Kamoshita A, Wade J, Ali L,et al. Mapping QTLs for root morphology of a rice population adapted to rainfed lowland conditions. Theor Appl Genet,  2002, 104(5): 880893.

\[18\]Qu Y, Mu P, Zhang H,et al. Mapping QTLs of root morphological traits at different growth stages in rice. Genetica,  2008, 133(2): 187200.

\[19\]胡兴明, 郭龙彪, 曾大力, 等. 水稻苗期发根力的QTL 和上位性分析. 中国水稻科学, 2004, 18(5): 396400.

\[20\]Jia L Q, Zhang B T, Mao C Z, et al. OsCYTINV1 for alkaline/neutral invertase is involved in root cell development and reproductivity in rice (Oryza sativa L.). Planta,  2008, 228(1): 5159.

\[21\]Nakamura A,Umemura I, Gomi K, et al. Production and characterization of auxininsensitive rice by overexpression of a mutagenized rice IAA protein. Plant J, 2006, 46(2): 297306.

\[22\]Zhu Z X, Liu Y, Liu S J,et al. A Gainoffunction mutation in OsIAA11 affects lateral root development in rice. Mol Plant,  2012, 5(1): 154161.

\[23\]Kitomi Y, Inahashi H, Takehisa H, et al. OsIAA13mediated auxin signaling is involved in lateral root initiation in rice. Plant Sci, 2012, 190: 116122.

\[24\]Ni J, Wang G H, Zhu Z X, et al. OsIAA23mediated auxin signaling defines postembryonic maintenance of QC in rice. Plant J,  2011, 68(3): 433442.

\[25\]Inukai Y, Sakamoto T, UeguchiTanaka M, et al. Crown rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin signaling. Plant Cell,  2005, 17(5): 13871396.

\[26\]Kitomi Y, Ogawa A, Kitano H, et al. CRL4 regulates crown root formation through auxin transport in rice. Plant Root,  2008, 2: 1928.

\[27\]Kitomi Y, Ito H, Hobo T, et al. The auxin responsive AP2/ERF transcription factor CROWN ROOTLESS5 is involved in crown root initiation in rice through the induction of OsRR1, a typeA response regulator of cytokinin signaling.  Plant J, 2011, 67(3): 472484.

\[28\]Wang X F, He F F, Ma X X, et al. OsCAND1 is required for crown root emergence in rice. Mol Plant,  2011, 4(2): 289299.

\[29\]Ding W, Yu Z, Tong Y, et al. A transcription factor with abHLH domain regulates root hair development in rice. Cell Res,  2009, 19(11): 13091311.

\[30\]You T, Toyota M, Ichii M, et al. Molecular cloning of a root hairless gene rth1 in rice. Breeding Sci,  2009, 59(1): 1320.

\[31\]Qi Y, Wang S, Shen C, et al. OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa). New Phytol,  2012, 193(1): 109120.

\[32\]Yao S G, Kodama R, Wang H,et al. Analysis of the rice SHORTROOT5 gene revealed functional diversification of plant neutral/alkaline invertase family. Plant Sci,  2009, 176(5): 627634.

\[33\]Uga Y, Sugimoto K, Ogawa S, et al. Control of root system architecture by DEEPER ROOTING 1 increases rice yield under drought conditions. Nat Genet,  2013, 45: 10971102.

\[34\]Wang S,Basten C J, Zeng Z B. Windows QTL Cartographer 2.5. Raleigh N C: Department of Statistics, North Carolina State University. \[20100201\]. http://statgen.ncsu.edu/qtlcart/WQTLCart. htm.

\[35\]Vision T J, Brown D G, Shmoys D B, et al. Selective mapping: a strategy for optimizing the construction of highdensity linkage maps. Genetics, 2000, 155(1): 407420.

\[36\]周广生, 崔克辉, 靳德明, 等. 发根力作为栽培稻品种苗期抗旱性鉴定指标的研究. 中国农业科学, 2005, 38(12): 25712576.

\[37\]刘开顺. 解决机插秧苗返青期长的技术措施. 农机科技推广, 2011, 3: 53.

\[38\]Kobayashi A, Koga Y,Uchiyamada H, et al. Breeding a new rice variety “Habataki”. Bull Hok Natl Agric Exp Stat,  1990, 32: 6584.
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