Two mutants with rolled leaves, derived from the indica cultivar 9311 via the radiation of 60Coγ ray in M2 generation, temporally designated as rl3(t)1 and rl3(t)2, were served as materials for exploring the mechanism underlying the rolling leaf characteristic. Morphological analysis showed that, these two mutants have typically adaxially rolled leaves. In addition, when compared with wild type 9311, the plant heights and panicle lengths of rl3(t)1 and rl3(t)2 significantly decreased, as well as the seed setting percentage. Cytological analysis suggests that the rolled leaf phenotype may be caused by the change of number and size of bulliform cells. Genetic analysis indicated that rolled leaf character is controlled by a recessive nuclear gene. Crossing between the two mutants, the F1\[rl3(t)1/rl3(t)2\] plants exhibit rolled leaf, suggesting they are allelic. To map the RL3(t) gene, an F2 population was generated by crossing the rl3(t)1 mutants with Wuyunjing 8 as a mapping population. By using simple sequence repeat (SSR) markers and some new designed sequence tagged site (STS) markers, RL3(t) was initially mapped in the region between the STS marker M345 and the SSR marker RM6676 with the genetic distances of 5.5 cM and 4.4 cM, respectively, on the long arm of chromosome 3. Furthermore, with the enlarged population and more developed STS markers, RL3(t) gene was finally delimited to a 46kb long region governed by the STS markers S339 and S336.
GUO Min, LI Rongde, YAO Jian, ZHU Juan, FAN Xiangyun, WANG Wei, TANG Shuzhu, GU Minghong, YAN Changjie*
. Fine Mapping of Gene RL3(t), Responsible for Leaf Shape Formation in Rice[J]. Chinese Journal OF Rice Science, 2014
, 28(5)
: 458
-464
.
DOI: 10.3969/j.issn.1001-7216.2014.05. 002
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