The homeobox transcription factors play important roles in the growth and development of the plants, involved in regulation of cell differentiation, the architecture of morphogenesis and response to environmental signals in plants. To study the function of these transcription factors in rice, we constructed RNAi vectors of OsHox9 and analyzed the function of OsHox9 by the reverse genetics method. The results showed that the height and tiller number of RNAi transgenic plants were decreased in comparison with wild type plants. Realtime PCR analysis showed that OsHox9 expression level was downregulated in transgenic plants with phenotype. However, the expression level of OsHox9 in transgenic plants without phenotype was similar to wild type plants, suggesting that the phenotypes of transgenic plants were caused by RNAi effects. In addition, we also analyzed the expression pattern of OsHox9 in different organs of rice. The results showed that OsHox9 was expressed in different organs, especially displayed high expression level in stem apical meristems and young panicles. To investigate the subcellular localization of OsHox9, we constructed the fused vector 35S::OsHox9GFP to introduce into leaves of tobacco. The transient expression result showed OsHox9 was localized on the cell membrane.
AI Liping, SHEN Ao, GAO Zhichao, LI Zhenglong, SUN Qionglin, LUAN Weijiang*
. Reverse Genetics Analysis of the Transcription Factor OsHox9, a Member of Homeobox Family, in Rice[J]. Chinese Journal OF Rice Science, 2014
, 28(3)
: 223
-228
.
DOI: 10.3969/j.issn.1001-7216.2014.03.001
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