PCRDGGE&nbsp; Analysis of the Bacterial Community in Different Populations of Rice Brown Planthopper, Nilaparvata lugens Stl

XU Hongxing1,2,  ZHENG Xusong2, YANG Yajun2, WANG Xin2, FU Qiang3, YE Gongyin1*,

doi:10.3969/j.issn.1001-7216.2014.02.015

2014 , Vol. 28 >Issue 2: 217 - 222

DOI: https://doi.org/10.3969/j.issn.1001-7216.2014.02.015

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PCRDGGE Analysis of the Bacterial Community in Different Populations of Rice Brown Planthopper, Nilaparvata lugens Stl

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¹ Institute of Insect Science, Zhejiang University Hangzhou 310058, China; ² Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; ³China National Rice Research Institute, Hangzhou 310006, China;

Received date: 2013-11-07

Revised date: 2014-01-23

Online published: 2014-03-10

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Abstract

The bacterial community structure in different virulent populations of brown planthopper (BPH), Nilaparvata lugens (Stl), was analyzed by denatured gradient gel electrophoresis (DGGE). The results showed that the bacterial community in BPH nymph from the 1st to the 5th instars varied with nymphal growth and development, and the bacterial community in the 1st instar BPH nymph was similar to that in adults. Nine geographic BPH populations were obviously divided into three groups according to the cluster analysis based on DGGE fingerprint. The first group came from the Philippines, the second from Thailand and the provinces of Hainan, Yunnan and Zhejiang of China, and the third from Vietnam and the provinces of Guangxi, Hunan, and Jiangxi of China. BPH populations on Mudgo (with resistant gene Bph1) and ASD7 (with resistant gene bph2) have similar bacterial community, while differed to that fed on susceptible rice variety, TN1.

Key words： denatured gradient gel electrophoresis (DGGE); brown planthopper (BPH); Nilaparvata lugens; bacterial community; geographic population; virulent to resistant varieties

Cite this article

XU Hongxing1,2, ZHENG Xusong2, YANG Yajun2, WANG Xin2, FU Qiang3, YE Gongyin1*, . PCRDGGE Analysis of the Bacterial Community in Different Populations of Rice Brown Planthopper, Nilaparvata lugens Stl[J]. Chinese Journal OF Rice Science, 2014 , 28(2) : 217 -222 . DOI: 10.3969/j.issn.1001-7216.2014.02.015

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