Six rice recombinant inbred lines with different heat tolerance and their parents, heat tolerant line 996 and heat sensitive line 4628 were treated with high temperature (9:00-17:00, 37℃; 17:00-9:00, 30℃) and optimal temperature (9:00-17:00, 30℃; 17:00-9:00, 25℃) in growth chambers. The effect of high temperature on antioxidant enzyme activities, membrane permeability,souble sugar content, heat stable protein content of anther, pollen grain and stigma viability were studied.The results showed the superoxide dismutase(SOD), catalase (CAT), peroxidase (POD) activities in anther increased obviously at early stage under high temperature stress, then decreased rapidly, the antioxidant enzyme activity of anther of heat tolerant lines was significantly higher than that of heat sensitive lines. Compared with heat sensitive lines, higher heat stable protein content and souble sugar content, lower malondialdehyde (MDA) content and relative conductivity were found in anther of heat tolerant lines. The anther dehiscence coefficient, pollen grains number on the stigma,pollen viability and stigma viability of heat tolerant lines were significantly higher than those of heat sensitive lines. It showed that hybrids might inherit the heat tolerance from the parent 996.The antioxidant enzyme activity, membrane permeability, heat stable protein content of anther and pollen viability can be used as heat resistance identification indexes in rice.
ZHANG Guilian1，2, ZHANG Shuntang1, XIAO Langtao3, TANG Wenbang1, XIAO Yinghui1, CHEN Liyun1,*
. Effect of High Temperature Stress on Physiological Characteristics of Anther,Pollen and Stigma of Rice During Headingflowering Stage[J]. Chinese Journal OF Rice Science, 2014
, 28(2)
: 155
-166
.
DOI: 10.3969/j.issn.1001-7216.2014.02.007
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