Research Papers

Identification and Genetic Mapping of a Thermosensitive White Leaf Mutant in Rice

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  • 1 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 2 Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;

Received date: 2013-11-15

  Revised date: 2013-12-12

  Online published: 2014-03-10

Abstract

A thermosensitive chlorophyll deficient mutant was obtained from the progeny of tissue culture of a japonica rice cultivar Asominori. The mutant was characterized by albino leaves before 3leaf stage under low temperature (23℃), while showing normal phenotype under high temperature(30℃). The chlorophyll contents of the mutant were significantly lower than those of wildtype before 3leaf stage under low temperature, which is due to altered chloroplast development under low temperature. Genetic analysis showed that the mutation was controlled by a single recessive gene, which is tentatively designed as cde2(chlorophyll deficient 2). Furthermore, using an F2 population derived from cde2/Peiai 64 and polymorphic markers between cde2 and Peiai 64, the cde2 was finally localized on the centromeric region of chromosome 1 and positioned between SSR markers RM11041 and Indel1, within the physical distance about 365.6 kb. In addition, the expression level of genes associated with chlorophyll biosynthesis, photosynthesis and plastidial transcription/translation apparatus was measured and the results shows that the cde2 mutation increased the transcript level of chlorophyll biosynthesis genes and the platidial transcription/translation apparatus related genes, but reduced the expression level of photosynthesis genes, indicating that CDE2 may involve in the chlorophyll biosynthesis and chloroplast development.

Cite this article

WEI Xiangjin1, #, SONG Jian1, 2, #, LIU Sheng1, SHAO Gaoneng1, SHENG Zhonghua1, TANG Shaoqing1 , HU Peisong1,* . Identification and Genetic Mapping of a Thermosensitive White Leaf Mutant in Rice[J]. Chinese Journal OF Rice Science, 2014 , 28(2) : 111 -118 . DOI: 10.3969/j.issn.1001-7216.2014.02.001

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