A molecular marker assisted selection (MAS) strategy was used to improve the resistance to rice stripe disease of Ninghui 8, the restorer line of japonica hybrid 86 you 8, by advanced backcross with Ninghui 8 as the recurrent parent and Kanto 194 as the donor of the rice stripe disease resistant gene. Markers tightly linked with Stvbi and Rf1a genes were used for selecting the two genes of the ability to recovery the sterility and to resistant the stripe disease, respectively. After identification of the rice stripe disease resistance and investigation of some agronomic traits, 10 resistance improved lines with similar characteristics as Ninghui 8 were developed in BC3F3 generation, and 4 lines with excellent characteristics were selected to cross with malesterile lines A25, A19 and A70, respectively. The results indicated that comprehensive characters of hybrid combinations from improved line2 were significantly better than those from Ninghui 8.
YAO Shu, CHEN Tao, ZHANG Yadong, ZHU Zhen, ZHAO Qingyong, ZHOU Lihui, ZHAO Ling, LI Yusheng, WANG Cailin*
. Improving Resistance to Rice Stripe Disease of Restorer Line Ninghui 8 by Molecular MarkerAssistant Selection[J]. Chinese Journal OF Rice Science, 2014
, 28(1)
: 85
-91
.
DOI: 10.3969/j.issn.1001-7216.2014.01.012
\[1\]程兆榜, 杨荣明, 周益军, 等. 江苏稻区水稻条纹叶枯病发生新规律. 江苏农业科学, 2002(1): 3941.
\[2\]王才林. 江苏省水稻条纹叶枯病抗性育种研究进展. 江苏农业科学, 2006(3): 15.
\[3\]Cheng S H, Cao L Y, Yang S H, et a1. Forty years’ development of hybrid rice: China’s experience. Rice Sci, 2004, 11(5/6): 225230.
\[4\]东丽. 杂交粳稻育种及配套技术科研质量管理研究. 沈阳: 沈阳农业大学, 2011.
\[5\]王才林. 江苏省杂交粳稻育种的现状、问题与对策. 西南农业学报, 2009, 22(4): 11651169.
\[6\]谷福林, 苏自强, 王水方. 优质杂交粳稻新组合86优8号. 杂交水稻, 2001, 16(2): 5960.
\[7\]李建卫, 潘国铭, 黄卫平. 杂交粳稻86优8号特征特性及其栽培技术. 中国稻米, 2003(5): 10.
\[8\]王才林, 张亚东, 朱镇, 等. 水稻条纹叶枯病抗性育种研究.作物学报, 2008, 34(3): 530533.
\[9\]姚姝, 张亚东, 朱镇, 等. 江苏省部分粳稻品种对条纹叶枯病的抗性鉴定及分子检测. 江苏农业学报, 2009, 25(6): 12011206.
\[10\]Dellapporta S L, Wood J, Hicks J B. A plant DNA mini preparation: VersionⅡ. Plant Mol Biol Rep, 1983, 1: 1921.
\[11\]Tsuji N. SCAR marker for selection of the rice stripe resistance gene Stvbi. Jpn Breeding Res, 2000(2): 6772.
\[12\]陈涛, 骆名瑞, 张亚东, 等. 粳稻BT型细胞质雄性不育恢复基因功能标记的开发与应用. 中国水稻科学, 2013, 27(3): 247258.
\[13\]Chen F, Temnykh S, Xu Y, et al. Development of a microsatellite framework map providing genome wide coverage in rice (Oryza sativa L.). Theor Appl Genet, 1997, 95: 553567.
\[14\]Washio O, Ezuka A, Sakurgi Y, et al. Studies on the breeding of rice varieties resistant to stripe disease: Varietal difference in resistance to rice stripe disease. Jpn J Breeding, 1967, 17(2): 9197.
\[15\]周彤, 周益军, 程兆榜, 等. 粳稻品种对水稻条纹叶枯病的抗性鉴定及抗病品种镇稻88的遗传分析. 植物保护学报, 2007, 34(5): 475479.
\[16\]王才林, 张亚东, 朱镇, 等. 通过分子标记辅助选择培育优良食味水稻新品种. 中国水稻科学, 2009, 23(1): 2530.
\[17\]姚姝, 陈涛, 张亚东, 等. 利用分子标记辅助选择聚合水稻暗胚乳突变基因Wxmq和抗条纹叶枯病基因Stvbi. 中国水稻科学, 2010, 24(4): 341347.