Research Papers

Changes in DNA Methylation Pattern in a Watersaving and Droughtresistance  Rice Variety at Threeleaf and Fourleaf stages after Drought Domestication

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  • 1 Shanghai Agrobiological Gene Center, Shanghai 201106, China; 2 College of Plant Sciences & Technology, Huazhong Agricultural  University, Wuhan 430070, China;

Received date: 2013-08-30

  Revised date: 2013-10-30

  Online published: 2014-01-10

Abstract

A watersaving and droughtresistance  rice Huhan 3 was grown under drought stress for  six successive generations after transplanting in the field. The variations in DNA methylation pattern between the original generation (G0) and the sixth generation (G6) were analyzed by methylation sensitive amplification polymorphism (MSAP) method. The results revealed that the methylated loci accounted for about 34% of the total. Among these, about 84% were fullmethylated loci and about 16% were hemimethylated ones. The DNA methylation level decreased from the threeleaf stage to the fourleaf stage in Huhan 3. Differentially methylated loci (DML) between generations or/and between different developmental stages accounted for 4.0% of the total, most of which were only related to plant development(579%). Compared to G0, the DNA methylation pattern of G6 changed after drought domestication, at threeleaf stage,  demethylated events occupying 59.1%, while at fourleaf stage, remethylated events occupied 47.9%. Genomewide alternations of DNA methylation were observed  along with rice growth and development, and DML mainly occurred on the gene′s promoter and exon region. The genes with DML were involved in a wide range of functions and participated in many important biological processes.

Cite this article

ZHENG Xiaoguo1,2,#, CHEN Liang1,#, LOU Qiaojun1, XIA Hui1, LI Mingshou1, LUO Lijun1,2,* . Changes in DNA Methylation Pattern in a Watersaving and Droughtresistance  Rice Variety at Threeleaf and Fourleaf stages after Drought Domestication[J]. Chinese Journal OF Rice Science, 2014 , 28(1) : 32 -40 . DOI: 10.3969/j.issn.1001-7216.2014.01.005

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