Using amylose content as the selection indicator, we chose the progenies with significantly different amylose content in grains by continuous and unidirectional orthoselection from F2 to F10 generations, compared the progeny of Dongnong 1006（Its grain amylose content was 18.82%）and Dongnong 1012（7.70%）with their parent Dongnong 423（18.33%）and Toukei 180（11.05%）, respectively. And we also compared the progeny of Dongnong 1006（18.82%）and Dongnong 1012（7.70%）with their parent Xixuan 1（18.74%）and Tong 769（17.05%）. Moreover, all groups above were chosen to analyze amylose accumulation and soluble starch synthase(SSS) activity and the expression levels of isoform genes during the grain filling. The results showed that the accumulated amount of amylose in parents and hybrid progeny with high amylose contents were always higher than that in the low amylose content progenies in different periods of grain filling, but for the amylopectin content, there were opposite results in the maturity stage. There were no significant negative correlations between the grain amylose content and amylopectin content 10, 17, 24 days after heading, and the correlation coefficients between SSS activity and the grain amylose content were 0.8115**, -07554*, -0.5957, respectively. In addition, the correlation coefficients between SSS activity and amylopectin content were -0.0694, 0.5453, -0.0207, respectively. The expression levels of the OsSSSⅠ,OsSSSⅡ1, OsSSSⅡ3, OsSSSⅢ1 and OsSSSⅢ2genes in endosperm of parents and hybrid progenies gradually peaked and then gradually declined, following a single peak curve along with the process of grain filling, while the expression levels of the OsSSSⅣ2 gene just showed a downdraft. However, the expression levels of the OsSSSⅢ2 and OsSSSⅣ2 genes were higher than others in various periods of grain filling. Compared with their parents, hybrid progeny with high amylose content have the expression of increased genes and reducing genes during the different period of filling, as well as the hybrid progeny with low amylose content compared with their parents. Moreover, there was significantly positive correlation between SSS activity and the expression levels of the OsSSSⅠ,OsSSSⅡ3,OsSSSⅢ2,and no significant positive correlation between SSS activity and the expression of the OsSSSⅡ1 and OsSSSⅣ2, and no significant negative correlation between SSS activity and the expression of the OsSSSⅢ1.
QU Ying, JIN Zhengxun*, LIU Haiying, XU Zhenhua, ZHU Linan, ZHENG Guanlong, ZHU Fangxu,
. Analysis of Expression Characters of Soluble Starch Synthase and Isoform Genes Involved in japonica Hybrid Progeny[J]. Chinese Journal OF Rice Science, 2014
, 28(1)
: 23
-31
.
DOI: 10.3969/j.issn.1001-7216.2014.01.004
\[1\]Reddy K R, Ali S Z, Bhattacharya K R. The fine structure of rice starch amylopectin and its relation to the texture of cooked rice. Carbohydr Polym, 1993, 22(4): 267275.
\[2\]程方民, 钟连进, 孙宗修. 灌浆结实期温度对早籼水稻籽粒淀粉合成代谢的影响. 中国农业科学, 2003, 36(5): 492501.
\[3\]Keeling P L, Bacon P J, Holt D C. Elevated temperature reduces starch deposition in wheat endosperm by reducing the activity of soluble starch synthase. Planta, 1993, 191(3): 342348.
\[4\]李木英, 石庆华, 胡志红, 等. 高温胁迫对不同早稻品种胚乳淀粉合成酶类活性的影响. 中国农业科学, 2007, 40(8):16221629.
\[5\]韦克苏, 张其芳, 程方民, 等. 花后高温下水稻可溶性淀粉合成酶同工型基因的表达模式. 作物学报, 2009, 35(1): 1824.
\[6\]吕冰, 郭志刚, 梁建生. 水稻胚乳中淀粉合成相关酶活性的变化对支链淀粉精细结构的影响. 中国科学C辑:生命科学, 2008, 38(8): 766773.
\[7\]沈鹏, 金正勋, 罗秋香, 等. 水稻灌浆过程中籽粒淀粉合成关键酶活性与蒸煮食味品质的关系. 中国水稻科学, 2006, 20(1): 5864.
\[8\]Zhao B H, Zhang W J. Changes in activities of the key enzymes related to starch synthesis in rice grains during grain filling and their relationships with the filling rate and cooking quality. Agric Sci China, 2005, 4(1): 2633.
\[9\]Manish K, Pandey N, Rani S. Different isoforms of starchsynthesizing enzymes controlling amylose and amylopectin content in rice (Oryza sativa L.). Biotechnol Adv, 2012, 30(6): 16971706.
\[10\]程方民, 蒋德安, 吴平, 等. 早稻籽粒灌浆过程中淀粉合成酶的变化及温度效应特征. 作物学报, 2001, 27(2): 201206.
\[11\]何照范. 粮油籽粒品质及其分析技术. 北京: 中国农业出版社, 1985: 137139.
\[12\]赵步洪, 张文杰, 常二华, 等. 水稻灌浆期籽粒中淀粉合成关键酶的活性变化及其与灌浆速率和蒸煮品质的关系. 中国农业科学, 2004, 37(8): 11231129.
\[13\]董明辉, 赵步洪, 吴翔宙, 等. 水稻结实期不同粒位籽粒相关内源激素含量和关键酶活性的差异及其与品质的关系. 中国农业科学, 2008, 41(2): 370380.
\[14 \]Hirose T, Terao T. A comprehensive expression analysis of the starch synthase gene family in rice(Oryza sativa L.). Planta, 2004, 220(1): 916.
\[15\]Denyer K, Smith A M. The purification and characterization of the two forms of soluble starch synthase from developing pea embryos. Planta, 1992, 186(4): 609627.
\[16\]Marshall J, Sidebottom C, Debet M. Identification of the major starch synthase in the soluble fraction of potato tubers. Plant Cell, 1996, 8(7): 11211135.
\[17\]Ohdan T, Francisco P B Jr， Sawada T, et al. Expression profiling of genes involved in starch synthesis in sink and source organs of rice. J Exp Bot, 2005, 56(422): 32293224.
\[18\]Baba T, Nishihara M, Mizuno K, et al. Identification, cDNA cloning, and gene expression of soluble starch synthase in rice (Oryza sativa L.) immature seeds. Plant Physiol, 1993, 103(2): 565573.
\[19\]朱军, 刘庆昌, 张天真, 等. 遗传学. 3版. 北京: 中国农业出版社, 2002: 312314.
\[20\]金正勋, 王露露, 刘海英, 等. 粳稻杂种后代胚乳淀粉分支酶基因表达特性分析. 东北农业大学学报, 2013, 44(1): 1823.
\[21\]刘海英, 李晓光, 刘洪亮, 等. 水稻杂种后代籽粒直链淀粉含量选择对品质和产量性状的影响. 中国水稻科学, 2009, 23(1): 107110.
\[22\]Vandeputte G E, Delcour J A. From sucrose to starch granule to starch physical behavior: A focus on rice starch. Carbohydr Polymer, 2004, 58(3): 245266.
\[23\]Nakamura Y. Towards a better understanding of the metabolic system for amylopeetin biosynthesis in plantsrice endosperm as a model tissue. Plant Cell Physiol, 2002, 43(7): 718–725.
\[24\]Commuri P D, Keeling P L. Chainlength specificities of maize starch synthase I enzyme: Studies of glucan affinity and catalytic properties. Planta, 2001, 25(5): 475486.
\[25\]Martha G, James Y, Kay D. Starch synthesis in the cereal endosperm. Curr Opin Plant Biol, 2003, 6(3): 215222.
\[26\]朱昌兰, 翟虎渠, 万建民. 稻米食味品质的遗传和分子生物学基础研究. 江西农业大学学报: 自然科学版, 2002, 24(4): 454
