Genetic Analysis and Gene Mapping of Pale Green Leaf Mutant HM14 in Rice

SHI Yongfeng＃, WEI Yanlin＃, FENG Baohua, WANG Huimei, XU Xia, HUANG Qina, LV Xiangguang, ZHANG Xiaobo, WU Jianli*

doi:10.3969/j.issn.1001-7216.2013.06.004

Chinese Journal OF Rice Science >

2013 , Vol. 27 >Issue 6: 585 - 590

DOI: https://doi.org/10.3969/j.issn.1001-7216.2013.06.004

Research Papers

Genetic Analysis and Gene Mapping of Pale Green Leaf Mutant HM14 in Rice

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State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; #These authors contributed equally to this paper;

Received date: 2013-06-27

Revised date: 2013-07-15

Online published: 2013-11-10

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Abstract

The palegreen leaf mutant HM14 was isolated from an EMSinduced IR64 mutant bank. The mutant showed a stable palegreen leaf phenotype throughout the whole life cycle. The plant height of HM14 decreased significantly while other agronomic traits were largely similar to the wildtype IR64. The chlorophyll content of HM14 reduced notably at tillering stage when compared with IR64. The net photosynthetic rate of HM14 was significantly lower than the wildtype IR64 at filling stage while the transpiration rate was raised significantly. Genetic analysis showed that the pale green leaf phenotype was controlled by a single recessive gene. To determine the location of the gene, bulked segregant analysis was carried out using both the mutant and wildtype DNA pools. The gene pglHM14 was finally delimited to a region of 299 kb between SSR markers RM1812 and RM26092 on the short arm of chromosome 11.

Key words： rice; pale green leaf mutant; chlorophyll content; gene mapping

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SHI Yongfeng＃, WEI Yanlin＃, FENG Baohua, WANG Huimei, XU Xia, HUANG Qina, LV Xiangguang, ZHANG Xiaobo, WU Jianli* . Genetic Analysis and Gene Mapping of Pale Green Leaf Mutant HM14 in Rice[J]. Chinese Journal OF Rice Science, 2013 , 27(6) : 585 -590 . DOI: 10.3969/j.issn.1001-7216.2013.06.004

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