Research Papers

Function of Phosphate Transporter OsPHT2;1 in Improving Phosphate Utilization in Rice

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  • College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China;

Received date: 2013-02-04

  Revised date: 2013-05-10

  Online published: 2013-09-10

Abstract

The uptake and distribution of Pi in plants requires multiple Pi transport systems throughout growth and development. A putative lowaffinity phosphate transporter gene, OsPHT2;1, was identified based on the rice genomic databases and bioinformatics analysis. The expression pattern of OsPHT2;1 in rice was investigated and the results showed its mRNA expressed in leaf mainly and in root little with the using of RTPCR. In addition, OsPHT2;1 induced by phosphate deficiency and light. By Agrobacterium tumefaciens transformation with an Ubiquitin (Ubi) promoter, the function of OsPHT2;1 was tested by overexpression in rice. Compared with wild type, the Pi concentrations of OsPHT2;1Oe plants increased by  40.9%-48.5% in leaves, 35.6%-51.2% in roots and the biomass increased by 25.1%-30.3% at Pisufficient levels. The Pi concentrations in OsPHT2;1Oe plants increased by 53.1%-70.3% in leaves and the biomass increased by 25.6%-28.5% at Pideficient levels, while the Pi concentrations had no difference in roots. In field test, the total P concentrations of OsPHT2;1Oe plants significantly increased in the top three leaves and panicle axis. It indicated that OsPHT2;1 might be involved in the Pi accumulation in leaves and Pi translocation in plants.

Cite this article

SHI Shulin#, WANG Danfeng#, YAN Yan, ZHANG Fang, WANG Huadun, GU Mian, SUN Shubin*, XU Guohua . Function of Phosphate Transporter OsPHT2;1 in Improving Phosphate Utilization in Rice[J]. Chinese Journal OF Rice Science, 2013 , 27(5) : 457 -465 . DOI: 10.3969/j.issn.1001-7216.2013.05.002

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