Improvement of Rice Bacterial Blight Resistance of Hybrid Rice Jinyou 207 by Molecular Markerassisted Selection

YAN Chengye, LIU Yan, MOU Tongmin*

doi:10.3969/j.issn.1001-7216.2013.04.005

Chinese Journal OF Rice Science >

2013 , Vol. 27 >Issue 4: 365 - 372

DOI: https://doi.org/10.3969/j.issn.1001-7216.2013.04.005

Research Papers

Improvement of Rice Bacterial Blight Resistance of Hybrid Rice Jinyou 207 by Molecular Markerassisted Selection

Expand

National Key Laboratory of Crop Improvement/National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China;

Received date: 2012-11-26

Revised date: 2012-12-10

Online published: 2013-07-10

Fold

Abstract

Two bacterial blight resistant varieties, Huahui 20 carrying Xa7 and Xa21 and CBB23 carrying Xa23 as donor parents were crossed with the male parent of hybrid rice Jinyou 207 as receptor parent. Nine bred lines carrying Xa7, Xa21, Xa23, Xa7 + Xa21, Xa7 + Xa23, respectively, under the genetic background of Xianhui 207 were selected by backcrossing, multicrossing and molecular markerassisted selection and pyramiding. Seven strains of Xanthomonas oryzae pv. oryzae, PXO61, PXO99, ZHE173, GD1358, FuJ, YN24 and HeN11 were inoculated to the nine bred lines and their hybrid combinations with Jin 23A as female parent for identifying the bacterial blight resistances. The results showed that bred lines and hybrid combinations carrying Xa7 gene were resistant to five Xoo strains, PXO61, ZHE173, GD1358, YN24 and FuJ. The bred line and their hybrid combination carrying Xa21 gene were resistant to six Xoo strains, PXO61, PXO99, ZHE173, GD1358, YN24 and FuJ. The bred lines and their hybrid combinations carrying Xa23, Xa7 + Xa21, Xa7 + Xa23 genes were resistant to all of the seven Xoo strains. The results showed that yield trials, main agronomic traits and rice quality of the two combinations, Jin 23A/CY100122 (carrying Xa7, resistance to five Xoo strains) and Jin 23A/CY100047 (carrying Xa7 and Xa21, resistant to seven Xoo strains) were similar to Jinyou 207. It can be concluded that these two combinations could replace Jinyou 207 for rice production of secondcropping in the Yangtze River Valley, which can increase the resistance level to bacterial blight.

Key words： hybrid rice; Jinyou 207; bacterial blight; molecular markerassisted selection

Cite this article

YAN Chengye, LIU Yan, MOU Tongmin* . Improvement of Rice Bacterial Blight Resistance of Hybrid Rice Jinyou 207 by Molecular Markerassisted Selection[J]. Chinese Journal OF Rice Science, 2013 , 27(4) : 365 -372 . DOI: 10.3969/j.issn.1001-7216.2013.04.005

References

\[1\]朱英国. 浅谈水稻事业与国家粮食安全. 中国乡镇企业, 2011(7): 4143.

\[2\]白辉, 李莉云, 刘国振. 水稻抗白叶枯病基因Xa21的研究进展. 遗传, 2006, 28(6): 745753.

\[3\]虞玲锦, 张国良, 丁秀文, 等. 水稻抗白叶枯病基因及其应用研究进展. 植物生理学报, 2012, 48(3): 223231.

\[4\]邓其明, 王世全, 郑爱萍, 等. 利用分子标记辅助育种技术选育高抗白叶枯病恢复系. 中国水稻科学, 2006, 20(2): 153158.

\[5\]章琦. 中国杂交水稻白叶枯病抗性的遗传改良. 中国水稻科学, 2009, 21(2): 111119.

\[6\]兰艳荣, 王俊义, 王弋, 等. 分子标记辅助选择改良水稻光温敏核不育系华201S的白叶枯病抗性. 中国水稻科学, 2011, 25(2): 169174.

\[7\]曾列先, 黄少华, 伍尚忠. IRBB21(Xa21)对广东稻白叶枯病菌5个小种的抗性反应. 植物保护学报, 2002, 29(2): 97100.

\[8\]姬广海, 张世光, 钱君. 云南水稻白叶枯病菌生理小种初析. 植物保护, 2003, 29(1): 1921.

\[9\]Singh S, Sidhu J S, Huang N, et al. Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using markerassisted selection into indica rice cultivar PR106. Theor Appl Genet, 2001, 102: 10111015.

\[10\]倪大虎, 易成新, 李莉, 等. 利用分子标记辅助选择聚合水稻基因Xa21和Pi9(t). 分子植物育种， 2005, 3(3): 329334.

\[11\]倪大虎, 易成新, 杨剑波, 等. 利用分子标记辅助选择聚合Pi9(t)和Xa23基因. 分子植物育种, 2007, 5(4): 491496.

\[12\]周宇爝, 邓其明, 李平. 利用MAS技术改良四个水稻恢复系及其抗病性分析. 分子植物育种, 2008, 6(3): 480490.

\[13\]Huang B, Xu J Y, Hou M S, et al.Introgression of bacterial blight resistance genes Xa7, Xa21, Xa22 and Xa23 into hybrid rice restorer lines by molecular markerassisted selection. Euphytica, 2012, 187(3): 449459.

\[14\]Xu J Y, Jiang J F, Dong X F, et al.Introgression of bacterial blight (BB) resistance genes Xa7 and Xa21 into popular restorer line and their hybrids by molecular markerassisted backcross (MABC) selection scheme. Afr J Biotechnol, 2012, 11(33): 82258233.

\[15\]王丹英, 邵国胜, 徐春梅, 等. 我国杂交水稻主栽品种的演变. 中国稻米, 2008(2): 13.

\[16\]中国水稻研究所国家水稻数据中心. http://www.ricedata.cn/variety/index.htm.

\[17\]Chen S, Huang Z H, Zeng L X, et al.Highresolution mapping and gene prediction of Xanthomonas oryzae pv. oryzae resistance gene Xa7. Mol Breeding. 2008, 22: 433441.

\[18\]潘海军, 王春莲, 赵开军, 等. 水稻抗白叶枯病基因Xa23的PCR分子标记及辅助选择. 作物学报, 2003, 29(4): 501507.

\[19\]高利军, 高汉亮, 李容柏, 等. 水稻抗白叶枯病基因Xa23分子标记的优化和验证. 分子植物育种, 2010, 8(4): 660664.

\[20\]Miyao A, Zhong H S, Monna L, et al. Characterization and genetic mapping of simple sequence repeats in the rice genome. DNA Res, 1996, 3(4): 233238.

\[21\]方中达, 许志刚, 过崇俭, 等. 中国水稻白叶枯病菌致病菌型的研究. 植物病理学报, 1990, 20(2): 8188.

\[22\]Mew T W. Current status and future prospects of research on bacterial blight of rice.Ann Rev Phytopathol, 1987, 25:359 382.

\[23\]Liu H, Wang Y, Hu B, et al. Virulence analysis and race classification of Xanthomonas oryzae pv. oryzae in China. J Phytopathol, 2007, 155(3): 129135.

\[24\]冯建成. 分子标记辅助选择技术在水稻育种上的应用. 中国农学通报, 2006, 22(2): 4347.

\[25\]王麒. 分子标记辅助选择及其在水稻育种中应用与展望. 黑龙江农业科学, 2009(1): 140143.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References