Research Papers

Transformation of γthionin Gene  RsAFP1 into Rice and Preliminary Idenfication of Its Blast Resistance

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  • 1 HighTech Research Center, Shandong Academy of Agricultural Sciences/Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Jinan 250100, China; 2  College of Life Sciences, Shandong Normal University, Jinan 250014, China;

Received date: 2012-10-31

  Revised date: 2012-12-03

  Online published: 2013-07-10

Abstract

A γthionin protein gene termed RsAFP1 isolated from the radish by PCR was introduced into the modified binary vector pCAMBIA1300. Six positive transformants were obtained by agrobacteriummediated transformation to rice callus. Further, the in vitro bacterial inhibition test and indoor rice blast fungus inoculation to rice seedling were conducted.In combination with the natural incidence in field, the resistance of transgenic lines were comprehensive evaluated. The results showed that introduction of RsAFP1 gene could improve rice blast resistance.

Cite this article

LI Haiqing1,2,#, LI Zhen1,#, WANG Yingying1, LIU Xu1, WANG Qingguo1, YAO Fangyin1, LIU Wei1,2,* . Transformation of γthionin Gene  RsAFP1 into Rice and Preliminary Idenfication of Its Blast Resistance[J]. Chinese Journal OF Rice Science, 2013 , 27(4) : 335 -343 . DOI: 10.3969/j.issn.1001-7216.2013.04.001

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