The insertion position of exogenous genes in targeted plant genomes are usually identified by adapter ligationmediated polymerase chain reaction, thermal asymmetric interlaced PCR, and restriction site extension PCR in transgenic rice research. However, there are various limitations in these methods, such as complexity of designing primers, and use of timeconsuming and multiplestep procedures. The goal of the present study was to establish an easier, and a more rapid and accurate method for cloning flanking sequence using singleprimer PCR in transgenic rice. Unknown flanking genome sequences in transgenic rice were successfully cloned using the singleprimer PCR method established in this study, with the gene as the anchor gene. Our data demonstrated that the singleprimer PCR is a more rapid and accurate method, justifying its application widely in cloning flanking sequences in transgenic rice.
LI Daoheng1, MA Jian1, WANG Yunpeng1, MA Jingyong1,*
. Cloning Flanking Sequence of Bar gene by Singleprimer PCR in Transgenic Rice[J]. Chinese Journal OF Rice Science, 2013
, 27(3)
: 321
-324
.
DOI: 10.3969/j.issn.10017216.2013.03.013
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