Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice

LI  Lei1,#, XUE Xiang1,#, ZUO Shimin1, CHEN Zongxiang1, ZHANG Yafang1, LI Qianqian1, ZHU Junkai2, MA Yuyin3, PAN Xuebiao1,*, PAN Cunhong2,*

doi:10.3969/j.issn.10017216.2013.03.001

Chinese Journal OF Rice Science >

2013 , Vol. 27 >Issue 3: 223 - 230

DOI: https://doi.org/10.3969/j.issn.10017216.2013.03.001

Research Papers

Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice

Expand

¹ Agricultural College, Yangzhou University, Yangzhou 225009, China; ² Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou 225007, China; ³ Department of Biochemical Engineering, Yangzhou Polytechnic College, Yangzhou 225009, China; #These authors contribute equally to this paper;

Received date: 2012-12-13

Revised date: 2013-01-24

Online published: 2013-05-10

Fold

Abstract

Argonaute (AGO) protein is the core effectors in RNAinduced silencing complexes (RISCs), playing an important role in plant development. The 2nd and the 11th-12th exon fragments of OsAGO1awere amplified independently to make two RNAi constructs: OsAGO1aI1E and OsAGO1aI2E. To clarify the function of OsAGO1a in rice development, the expression of OsAGO1a has been suppressed by introducing RNAi constructs to Nipponbare through Agrobacteriummediated transformation. The result showed that the reduced expression of OsAGO1a lead to adaxial leaf rolling without affecting main agronomic traits.

Key words： rice; OsAGO1a; polarity development; rolled leaf; agronomic traits

Cite this article

LI Lei1,#, XUE Xiang1,#, ZUO Shimin1, CHEN Zongxiang1, ZHANG Yafang1, LI Qianqian1, ZHU Junkai2, MA Yuyin3, PAN Xuebiao1,*, PAN Cunhong2,* . Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice[J]. Chinese Journal OF Rice Science, 2013 , 27(3) : 223 -230 . DOI: 10.3969/j.issn.10017216.2013.03.001

References

\[1\]Vaucheret H, Vazquez H, Crété P, et al. The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development. Genes Dev, 2004, 18:11871197.

\[2\]Song J J, Liu J, Tolia N H, et al. The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes. Nat Struct Biol, 2003, 10(12):10261032.

\[3\]Mi S J, Cai T, Hu Y G, et al. Sorting of small RNAs into Arabidopsis Argonaute complexes is directed by the 5’ terminal nucleotide. Cell, 2008, 133: 116127.

\[4\]Montgomery T A, Howell M D, Cuperus J T, et al. Specificity of ARGONAUTE7miR390 interaction and dual functionality in TAS3 transacting siRNA formation. Cell, 2008, 133:128141.

\[5\]Okamura K, Ishizuka A, Siomi H, et al. Distinct roles for Argonaute proteins in small RNAdirected RNA cleavage pathways. Genes Dev, 2004, 18:16551666.

\[6\]Liu J D, Carmell M A, Rivas F V, et al. Argonaute2 is the catalytic engine of mammalian RNAi. Science, 2004, 305:14371441.

\[7\]Meister G, Landthaler M, Patkaniowska A, et al. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol Cell, 2004, 15:185197.

\[8\]Baumberger N, Baulcombe D C. Arabidopsis ARGONAUTE1 is an RNA slicer that selectively recruits microRNAs and short interfering RNAs. Proc Natl Acad Sci, 2005, 102(33):1192811933.

\[9\]Sunkar R, Zhu J K. Novel and stressregulated microRNAs and other small RNAs from Arabidopsis. Plant Cell, 2004, 16: 20012019.

\[10\]Carrington J C, Ambros V. Role of microRNAs in plant and animal development. Science, 2003, 301: 336338.

\[11\]Kapoor M, Arora R, Lama T, et al. Genomewide identification, organization and phylogenetic analysis of Dicerlike, Argonaute and RNAdependent RNA polymerase gene families and their expression analysis during reproductive development and stress in rice. BMC Genom, 2008, 9: 451.

\[12\]Shi Z Y, Wang J, Wan X S, et al. Overexpression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erectleaf habit. Planta, 2007, 226: 99108.

\[13\]Wu L, Zhang Q, Zhou H, et al. Rice MicroRNA effector complexes and targets. Plant Cell Online, 2009, 21: 34213435.

\[14\]Thompson J D, Gibson T J, Plewniak F, et al. The CLUSTAL_X windows interface: Xexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 1997, 25: 48764882.

\[15\]Tamura K, Dudley J, Nei M, et al. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4. 0. Mol Biol Evol, 2007, 24:15961599.

\[16\]Hiei Y, Ohta S, Komari T, et al. Efficient transformation of rice (Oryza sativa L. ) mediated by Agrobacterium and sequence analysis of the boundaries of the TDNA. Plant J, 1994, 6(2):271282.

\[17\]Xie Z, Kasschau K D, Carrington J C. Negative feedback regulation of DicerLike1 in Arabidopsis by microRNAguided mRNA degradation. Curr Biol, 2003, 13:784789.

\[18\]Zhong R, Ye Z H. Regulation of HDZIP III genes by microRNA 165. Plant Signal Behav, 2007, 2(5): 351353.

\[19\]Zhou G K, Kubo M, Zhong R, et al. Overexpression of miR165 affects apical meristem formation, organ polarity establishment and vascular development in Arabidopsis. Plant Cell Physiol, 2007, 48(3): 391404.

\[20\]Williams L, Grigg S P, Xie M, et al. Regulation of Arabidopsis shoot apical meristem and lateral organ formation by microRNA miR166g and its AtHDZIP target genes. Development, 2005, 132(16): 36573668.

\[21\]Emery J F, Floyd S K, Alvarez J, et al. Radial patterning of Arabidopsis shoots by class III HDZIP and KANADI genes. Curr Biol, 2003, 13(20):17681774.

\[22\]Mallory A C, Reinhart B J, JonesRhoades M W, et al. MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5’ region. Embo J, 2004, 23(16):33563364.

\[23\]Juarez M T, Kui J S, Thomas J, et al. MicroRNAmediated repression of rolled leaf1 specifies maize leaf polarity. Nature, 2004, 428: 8488.

\[24\]邵元健, 潘存红, 陈宗祥, 等. 水稻不完全隐性卷叶主基因rl(t)的精细定位. 科学通报, 2005, 50(19): 21072113.

\[25\]潘存红, 李磊, 陈宗祥, 等. 一个水稻卷叶基因rl(t)的精细定位. 中国水稻科学, 2011, 25: 455460.

\[26\]杨守仁, 张龙步, 王进民. 水稻理想株型育种的理论和方法初论. 中国农业科学, 1984, 15(3): 613.

\[27\]周开达, 马玉清, 刘太清, 等. 杂交水稻亚种间重穗型组合选育杂交水稻超高产育种的理论与实践. 四川农业大学学报, 1995, 13(4): 403407.

\[28\]袁隆平. 杂交水稻超高产育种. 杂交水稻, 1997, 12(6): 16.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References