A rice chromosome single segment substitution line(SSSL) S42, which is a strong restorer line for wild abortive cytoplasmic male sterility(WACMS) and YCMS, was identified at the Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University. The genetic mode of fertility restorer(Rf) genes and the genetic relationship between WACMS and YCMS systems were characterized through the production of backcross populations. The SSSL S42 was used to pollinate WACMS line of Zhenshan 97A and YCMS line of YHuanong A and generated two BC3F2 populations by using markerassisted selection and traditional backcrossing. Results were shown as the following: 1) In the genetic background of S42, WACMS was restored more easily than YCMS, and the effect of Rf4 appeared to be slightly larger than that of Rf3 for the two CMS systems; 2) Two pairs of dominant genes Rf3 and Rf4 governed pollen fertility restoration, and modifying or minor genes also involved in the inheritance of restorer ability in S42, which indicates that the genetic mode of Rf genes was a qualitativequantitative character for WACMS and YCMS systems; 3) When 205 SSR markers were used to analyze the genetic background and average length substituted chromosome segments(SCS) of the two BC3F2 individuals, the number of SCS carried by BC3F2 individuals with the genotypes of Rf3Rf3/rf4rf4 or rf3rf3/Rf4Rf4, was 1.1, while the average lengths of the SCS, corresponding to Rf3 and Rf4 loci, were 14.5 cM and 17.4 cM, respectively.
\[1\]高明尉. 野败型杂交籼稻基因型的初步分析. 遗传学报, 198l, 8(1): 6674.
\[2\]杨仁崔, 卢浩然. 水稻恢复系IR24恢复基因的初步分析. 作物学报, 1984, 10(2): 8186.
\[3\]张桂权, 卢永根. 水稻质核互作孢子体雄性不育性的基因分析. 作物学报, 1987, 13(1): 2328.
\[4\]Zhang G, Lu Y, Huang N. Molecular analysis of introgressed chromosomal segments in a set of nearisogenic lines of rice for fertility restoring genes. Rice Genet Newsl, 1994, 11: 147149.
\[5\]Zhang G, Bharaj T S, Virmani S S, et al. Mapping of the Rf3 nuclear fertilityrestoring gene for WA cytoplasmic male sterility in rice using RAPD and RFLP markers.Theor Appl Genet, 1997, 94: 2733.
\[6\]Yao F Y, Xu C G, Yu S B, et al. Mapping and genetic analysis of two fertility restorer loci in the wild abortive cytoplasmic male sterility system of rice(Oryza sativa L.). Euphytica, 1997, 98:183187.
\[7\]Tan X L, Vanavichit S, Amornsilpa S, et al. Genetic analysis of rice CMSWA fertility restoration based on QTL mapping. Theor Appl Genet, 1998, 96: 994999.
\[8\]庄杰云, 樊叶杨, 吴建利, 等．水稻CMSWA育性恢复基因的定位．遗传学报, 2001, 28（2）： 129134.
\[9\]张群宇, 刘耀光, 张桂权, 等. 野败型水稻细胞质雄性不育恢复基因Rf4的分子标记定位. 遗传学报, 2002, 29(11): 10011004.
\[10\]Sattari M, Kathiresan A, Glenn B, et al. Comparative genetic analysis and molecular mapping of fertility restoration genes for WA,Dissi, and Gambiaca cytoplasmic male sterility systems in rice. Euphytica, 2008, 160: 305315.
\[11\]Sheeba N K, Viraktamath B C, Sivaramakrishnan S, et al. Validation of molecular markers linked to fertility restorer gene(s) for WACMS lines of rice. Euphytica, 2009, 167: 217227.
\[12\]Ngangkham U, Parida S K, De S, et al. Genic markers for wild abortive(WA) cytoplasm based male sterility and its fertility restoration in rice.Mol Breeding, 2010, 26: 275292.
\[13\]蔡善信. 华南晚籼夜公细胞质(Y型) 雄性不育系的选育. 中国水稻科学, 2002, 16(2): 185188.
\[14\]Zhang G Q, Zeng R Z, Zhang Z M, et al. The construction of a library of single segment substitution lines in rice(Oryza sativa L.). Rice Genet Newsl, 2004, 21: 8587.
\[15\]Xi Z Y, He F H,Zeng R Z, et al. Development of a wide population of chromosome singlesegment substitution lines in the genetic backgroundof an elite cultivar of rice(Oryza sativa L.). Genome, 2006, 49: 476484.
\[16\]廖秋平. 水稻(Oryza sativa L.) 细胞质雄性不育恢复性的等位基因分化\[硕士学位论文\]. 广州: 华南农业大学, 2008.
\[17\]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8(19): 43214325.
\[18\]Li W T, Zeng R Z, Zhang Z M, et al. Mapping of Sb locus for F1 pollen steril ity in cultivated rice using PCR based markers. Acta Bot Sin, 2002, 44(4): 463467.
\[19\]McCouch R, Teytelman L, Xu Y B, et al. Development and mapping of 2240 new SSR markers for rice(Oryza sativa L.). DNA Res, 2002, 9: 199207.
\[20\]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism(SSPL) in rice(Oryza sativa L.). Mol Gen Genet, 1996, 252: 597607.
\[21\]Singh A K, Mahapatra T, Prabhu K V, et al. Application of molecular markers in rice breeding: progress at IARI//Advances in Markerassisted Selection Workshop. Trainee’s manual, Handouts and references, 2005.
\[22\]张桂权, 卢永根. 栽培稻(Oryza sativa L.) 杂种不育性的遗传研究: I.等位基因 F1不育系杂种不育性的双列分析. 中国水稻科学, 1989, 3(3): 97101.
\[23\]Young N D, Tanksley S D. Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet, 1989, 77: 95101.
\[24\]黄朝锋. 水稻 PSM 标记的发展及抗虫基因的分子定位\[学位论文\]. 广州: 华南农业大学, 2003.
\[25\]Kubo T, Aida Y, Nakamura K, et al. Reciprocal chromosome segment substitution series derived from japonica and indica cross of rice(Oryza sativa L.). Breed Sci, 2002, 52: 319325.
\[26\]Ebitani T, Takeuchi Y, Nonoue Y, et al. Construction and evaluation of chromosome segment substitution lines carrying overlapping chromosome segments of indica rice cultivar ‘Kasalath’ in a genetic background of japonica elite cultivar ‘Koshihikari’. Breed Sci, 2005, 55: 6573.
\[27\]张丽霞, 刘丕庆, 刘学义, 等. 染色体单片段代换系的构建及应用于QTL精细定位. 分子植物育种, 2(3): 743746.
\[28\]刘冠明, 李文涛, 曾瑞珍, 等. 水稻亚种间单片段代换系的建立. 中国水稻科学, 2003, 17(3): 201204.
\[29\]何风华, 席章营, 曾瑞珍, 等. 利用高代回交和分子标记辅助选择建立水稻单片段代换系. 遗传学报, 2005, 32(8): 825831.
\[30\]He F H, Xi Z Y, Talukdar A, et al. Identification of QTLs for plant height and its components by using single segment substitution lines in rice(Oryza sativa L.). Rice Sci, 2005, 12(3): 151156.
\[31\]李文涛, 曾瑞珍, 张泽民, 等. F1花粉不育性近等基因系导入片段的分析. 中国水稻科学, 2003, 17(2): 9599.
\[32\]Bharaj T S, Virmani S S, Khush G S. Chromosomal location of fertility restoring genes for wild abortive cytoplasmic male sterility using primarytrisomics in rice. Euphytica, 1995, 83: 169173.
\[33\]徐才国, 唐为江, 邢永忠. 水稻优良恢复系明恢63的两个恢复基因恢复力的单独评价. 分子植物育种, 2003, 1(4): 497501.
\[34\]滕利生, 申宗坦. 水稻胞质不育的恢复基因分析. 作物学报, 1996, 22(2): 142146.
\[35\] 黄青阳, 何予卿, 凌杏元, 等. 3种水稻细胞质雄性不育系育性遗传的比较. 中国农业科学, 2000, 33(3): 813.
\[36\]Tao D Y, Xu P, Li J, et al. Inheritance and mapping of male sterility restoration gene in upland japonica restorer lines.Euphytica, 2004, 138: 247254.
