OsIP1, encoding soluble inorganic pyrophosphatase was cloned and inserted between the rice mesophyllspecific promoter “cyFBPase” and the poly(A) site of the octopine synthase gene, and transferred into different sourcesink type rice cultivars, including sourcelimiting type Zhongchao 123 and sinklimiting type Nongken57 by Agrobacteriummediated gene transfer. Some T0 transgenic plants with high expression level were selected by quantitative realtime PCR and their T2 homozygous lines with the same morphological and agronomic traits as their wildtype cultivar were generated. The effects of overexpressed OsIP1 specifically in the mesophyll cell were estimated by investigating tiller dynamics, soluble and stored carbohydrate contents in leaf blade and leaf sheath, and dry weight and yield and so on. The preliminary results showed that during the vegetative stage, the tilling capability and panicles per plant of transgenic lines increased to a certain extent, especially in the high tillering capability variety, Nongken 57. The results also showed that during the grainfilling stage, the changing trends of the soluble sugar, sucrose and starch contents in leaf blade and leaf sheath of transgenic lines were consistent with those of the wild types. Except leaf sheath at the peak grainfilling stage, the sucrose content of transgenic lines were increased significantly at each time point. Compared with the wild types, the dry weights and grain yields of transgenic lines increased to a varying degree. The grain yield of the transgenic lines of Zhongchao 123 were significantly higher than that of the wild type.
ZHANG Yafang, CHEN Zongxiang, ZUO Shimin, LOU Lijuan, YU Yongqi, PAN Xuebiao
. Effect of Overexpressing OsIP1, a Rice Inorganic Pyrophosphatase Gene, Specifically in Mesophyll Tissue of Different Sourcesink Type Rice Cultivars[J]. Chinese Journal OF Rice Science, 2013
, 27(2)
: 129
-136
.
DOI: 10.3969/j.issn.10017216.2013.02.004
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