To verify the direct effect of H+ATPase on the root cell elongation, the T2 generation seeds of the rice plasma membrane H+ATPase gene OsA8 (AJ440219) overexpressed transgenic materials (OE1) were used as samples. Firstly, the differences in seed germination rate and the growth rate of primary roots between the overexpressed line and the wildtype were determined. Secondly, the activities of H+ATPase in the root cell membranes isolated by twophase separation method were compared and then the OsA8 gene expression at the transcriptional level was determined. The protein concentration of H+ATPase of these two materials was analyzed by Western blot. The results showed that not only the germination rate but also the growth rate of primary roots of OE1 were higher than that of wildtype. In addition, OE1 has much higher activity of the root plasma membrane H+ATPase than wildtype. The expression of OsA8 gene and protein concentration of OE1 was also higher than that of wildtype. The results indicated that the overexpression of the plasma membrane H+ATPase gene OsA8 in the OE1 rice is the main factor of the elevation of the H+ATPase activity, which contributed to the increased seed germination rate and growth of primary root.
LIU Gan1, ZHANG Mingchao1, ZENG Houqing1, 2, ZHU Yiyong1, *, SUN Shubin1, XU Guohua1
. Overexpression of a Plasma Membrane H+ATPase Gene Promotes Rice Seed Germination[J]. Chinese Journal OF Rice Science, 2012
, 26(6)
: 651
-655
.
DOI: 10.3969/j.issn.10017216.2012.06.003
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