Salttolerance Enhancement of Transgenic Rice with Na+/H+ Antiporter Gene Driven by Rootspecific Promoter PmPgPR10

WU  Limin1, CHEN Wei1, ZHAO Yan2, FENG Shangguo1, YING Qicai1, LIU Junjun3, WANG Huizhong1，*

doi:10.3969/j.issn.10017216.2012.06.002

Chinese Journal OF Rice Science >

2012 , Vol. 26 >Issue 6: 643 - 650

DOI: https://doi.org/10.3969/j.issn.10017216.2012.06.002

Research Papers

Salttolerance Enhancement of Transgenic Rice with Na+/H+ Antiporter Gene Driven by Rootspecific Promoter PmPgPR10

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¹ Key Laboratory of Hangzhou City for Biochemistry and Molecular Biology, Hangzhou Normal University, Hangzhou 310036, China; ² College of Food and Bioengineering, Zhejiang Gongshang University, Hangzhou 310035, China; ³ Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Rd, Victoria, British Columbia, V8Z 1M5, Canada;

Received date: 2012-03-06

Revised date: 2012-04-19

Online published: 2012-11-10

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Abstract

To obtain the saltresistant rice varieties, Na+/H+ antiporter gene was cloned from Triticum aestivum (TaNHX2) and transformed into rice driven by rootspecific promoter of PR10 gene from Pinus griffithii (PmPgPR10∷TaNHX2), which hold the classical promoter features like TATAbox motif. Results of PCR, Southern and realtime PCR showed that the target gene had been integrated into rice genome successfully. The salttolerance assay showed that the salttolerance of the PmPgPR10∷TaNHX2 transgenic rice lines was significantly increased compared with nontransgenic rice. This result was consistent with the enhancement of the expression level of TaNHX2 gene under salt stress and indicated that the PmPgPR10 promoter was able to regulate the rootspecific expression of TaNHX2. To further explore the salttolerance mechanism of transgenic lines, the activity of VATPase and VPPase were measured and the higher enzyme activity in PmPgPR10∷TaNHX2 transgenic line was attributed to the healthy growth status under salt stress. Furthermore, the activity improvement of the VATPase and VPPase were detected under salinity stress only in root, not in leaf, further indicating the rootspecific function of PmPgPR10 promoter. Based on those results, we conclude that the PmPgPR10 promoter from Pinus griffithii has the strong ability to enhance the expression of downstream TaNHX2 gene in rice to resist the salt damage.

Key words： promoter; Pinus griffithii; Oryza sativa L.; Na+/H+ antiporter

Cite this article

WU Limin1, CHEN Wei1, ZHAO Yan2, FENG Shangguo1, YING Qicai1, LIU Junjun3, WANG Huizhong1，* . Salttolerance Enhancement of Transgenic Rice with Na+/H+ Antiporter Gene Driven by Rootspecific Promoter PmPgPR10[J]. Chinese Journal OF Rice Science, 2012 , 26(6) : 643 -650 . DOI: 10.3969/j.issn.10017216.2012.06.002

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