Phytochrome family in rice is composed of three members, PHYA, PHYB, and PHYC. Through characterizing the phytochrome mutants and wild type (WT) in terms of photomorphogenesis, roles of individual phytochromes have been preliminarily explored in regulating rice deetiolation, flowering time and fertility. We compared the chlorophyll contents of wild type and phyA, phyB and phyAphyB mutants grown either in white light (W) or in red light (R). The results suggest that phyB perceives red light to positively regulate chlorophyll biosynthesis, while the role of phyA can be detected only in the phyBdeficient mutant. Analyses on the expression levels of genes involved in chlorophyll biosynthesis revealed that phytochromes affected chlorophyll biosynthesis by regulating protochlorophyll oxidoreductase A(PORA ) expression. The role of phyB in chloroplast development was analyzed. The results suggest that phyB perceive red light to regulate chloroplast development by affecting the number of chloroplasts and grana, as well as the chloroplast membrane system.
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