To investigate the cytogenotoxicity of Bt63, a genetically modified(GM) rice in human lymphocytes in vitro, human lymphocytes were exposed to whole protein of Bt63 at doses of 200, 100, 50, 25, 12.5 μg/mL and incubated for 1.5 h, 6 h and 24 h, respectively. The DNA damage, chromosomal aberrations and cytotoxicity induced by whole protein of Bt63 were measured by comet assay, micronucleus test, CCK8 assay and neutral red uptake (NRU) assay. After different incubation periods, the cytogenotoxicity of lymphocytes in positive control group was significant higher than that of lymphocytes in blank control group. Moreover, the exposure timeeffect relationship was observed in positive control group by CCK8 assay and neutral red uptake (NRU) assay. There was no significant difference in cytogenotoxicity between Bt63 group and nonGM rice group. Also, the Bt63 GM rice group did not show higher cytogenotoxicity than that of nonGM rice group, indicating that Bt63 GM rice has similar cytogenotoxicity with nonGM rice, and Bt63 GM rice has no cytogenotoxicity effect on human lymphocytes in vitro. Compared with traditional toxicological methods, the experimental system is economic, simple, rapid and reliable.
CHEN Wujian1, ZHENG Wei2,*, LU Qinfeng2, ZHANG Mingzhe1, WU Zhonghua2, XU Qi2, WU Zhiyi1, LI He2
. Safety Assessment of Bt63, a Genetically Modified Rice in vitro[J]. Chinese Journal OF Rice Science, 2012
, 26(5)
: 633
-636
.
DOI: 10.3969/j.issn.10017216.2012.05.019
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