A fragment of 1 184 bp of the Ospgip1 gene including a complete open reading frame (OFR) of 930 bp had been amplified, which had the termination codon of TAA and no intron. RTPCR results showed that the Ospgip1 gene could be expressed in all the rice cultivars while their expression levels differed among growth stages or organs. Realtime PCR results indicated that the expression levels in resistant cultivars YSBR1 and Jasmine 85 were significantly higher than that in the susceptible cultivar Lemont. The expression level was also upregulated in the etiolated seedlings. Inoculation with the pathogen of rice sheath blight significantly upregulated the expression level of the Ospgip1 gene in resistant cultivars while exerted no impact on the expression level of the gene in the susceptible one.
CHEN Xijun1, 2, LIU Xiaowei1, ZUO Shimin2, TONG Yunhui1, PAN Xuebiao2,*, XU Jingyou1，*
. Expression Characteristics of the Ospgip1 Gene in Rice Cultivars with Resistance or Susceptiblility to Sheath Blight[J]. Chinese Journal OF Rice Science, 2012
, 26(5)
: 629
-632
.
DOI: 10.3969/j.issn.10017216.2012.05.018
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