Impact of Continuous Cross Inoculation on Variation of Xanthomonas oryzae pv.&nbsp;&nbsp; oryzae from japonica Rice in Yunnan Plateau

YANG  Yayun1，3,4,#, PENG Xinxi1,2，#, XU  Furong1,3,4, HE Jianping1,2, A Xinxiang1,3,4, DONG Chao1,3,4, ZHANG Enlai1,3,4,  ZHANG Feifei1，3,4, TANG Cuifeng1,3,4, DAI  Luyuan1,3,4,*

doi:10.3969/j.issn.10017216.2012.05.011

2012 , Vol. 26 >Issue 5: 583 - 591

DOI: https://doi.org/10.3969/j.issn.10017216.2012.05.011

Research Papers

Impact of Continuous Cross Inoculation on Variation of Xanthomonas oryzae pv. oryzae from japonica Rice in Yunnan Plateau

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¹ Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, China; ² School of Life Sciences of Yunnan University, Kunming 650091, China;³ Yunnan Provincial Key Laboratory of Agricultural Biotechnology；⁴ Key Laboratory of Southwestern Crop Gene Resource and Germplasm Innovation, Ministry of Agriculture； #These authors contributed equally to this paper；

Received date: 2011-08-23

Revised date: 2011-11-12

Online published: 2012-09-10

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Abstract

To study the factors influenceing variation of Xanthomonas oryzae pv. oryzae( Xoo) from japonica rice in Yunnan plateau, eight strains(30 isolates) derived from two original strains 20012 and K1 were obtained through successivecross inoculation.Their pathotype and gene variation were studied by using differential cultivars and molecular detection methods. The results showed that seven obtained strains including 23 isolates observably differed with the original isolate 20012 in pathotype, beside the pathogenicity of 62 containing 3 isolates were increased, while the pathogenicity of the other six strains containing 20 isolates were decreased. The pathotypes of 68 were the same as that of its original isolate K1. By using UPGMA analysis of PCR producets of primer IS1112 and hrpF, no relationship between molecular haplotypes and pathotypes were found. Sequence analysis showed that basic group variation in sequences of all strains amplified by primers IS1112 and hrpF differed. The pathogenic variation of obtained strains from two original strains inoculated on various varieties were unique and nondirective.

Key words： Yunnan plateau; Xanthomonas oryzae pv.oryzae; continuous cross inoculation; variation

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YANG Yayun1，3,4,#, PENG Xinxi1,2，#, XU Furong1,3,4, HE Jianping1,2, A Xinxiang1,3,4, DONG Chao1,3,4, ZHANG Enlai1,3,4, ZHANG Feifei1，3,4, TANG Cuifeng1,3,4, DAI Luyuan1,3,4,* . Impact of Continuous Cross Inoculation on Variation of Xanthomonas oryzae pv. oryzae from japonica Rice in Yunnan Plateau[J]. Chinese Journal OF Rice Science, 2012 , 26(5) : 583 -591 . DOI: 10.3969/j.issn.10017216.2012.05.011

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