Cloning of the major locus S5， a widecompatibility gene has laid a molecular basis for identification of the widecompatibility varieties(WCV). According to the difference in S5 locus sequence among indica, japonica and WCV, PCR was conducted and celery endonuclease CEL Ⅰ,which cleaves DNA with high specificity at sites of basesubstitution mismatch, was used to digest PCR products so as to distinguish various S5 DNA fragments. The results showed that the method is accurate in indicajaponica classification. At the same time, a new genotype on the S5 locus was found.
NI Shen1 , XIAO Wenfei2, CHEN Hongqi1, WANG Yuexing1, ZHU Xudong1，*
. Molecular Classification of indicajaponica Rice According to Widecompatibility Gene S5 Sequence with Endonuclease CEL Ⅰ [J]. Chinese Journal OF Rice Science, 2012
, 26(5)
: 549
-554
.
DOI: 10.3969/j.issn.10017216.2012.05.006
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