Conserved functional region of DUF500 was cloned by PCR from cDNA of expression HarpinXoo (hrf1) transgenic rice NJH12. OsDUF500 encodes an unknown function member of DUF500 family. Calli from mature embryos of rice cultivar R109 were transformed by Agrobacterium tumefaciens strain EHA105/ABD::pVec8. There were eight OsDUF500 transgenic lines from nine hygromycin resistant lines by PCR analysis. Compared with the wild type, the downexpressed lines ABD are characterized by reduced plant height, shortened leaf and white blighted grain. Disease assays of transgenic rice plants infected by Xanthomonas oryzae pv. oryzae (PXO99) indicated that different transgenic lines had different resistance to rice leaf blight. Lesion length of the downexpression lines reduced significantly and lesion area all bellowed 30%. Lesion areas of the ABD3 and ABD5 were 12.3% and 15.7%, respectively. Thus, we predicted OsDUF500gene, which was upregulated in rice blast, maybe was a negative regulator on bacterial blight resistance.
LI Juan, CHANG Shanshan, LIU Fengquan, SHAO Min*
. Silencing of OsDUF500 Gene in Rice Enhances Resistance to Xanthomonas oryzae pv. oryzae[J]. Chinese Journal OF Rice Science, 2012
, 26(4)
: 476
-480
.
DOI: 10.3969/j.issn.10017216.2012.04.013
\[1\]Swings J, van den Mooter M, Vauterin L, et al. Reclassification of causal agents of bacterial blight( Xanthomona campestris pv. oryzae) and bacterial leaf streak(Xanthomona campestris pv. oryzicola) of rice as pathovars of Xanthomona pv. oryzae. Int J Syst Bacteriol, 1990, 40: 309311.
\[2\]陈利锋,徐敬友.农业植物病理学.3版.北京:中国农业出版社,2007.
\[3\]Shao M, Wang J S, Dean B A,et al. Expression of a harpinencoding gene in rice confers durable nonspecific resistance to Magnaporthe grisea. Plant Biotechnol J,2008,6: 7381.
\[4\]邵敏,肖姗姗,李林等. 转hrf1基因水稻对稻瘟病多小种非专化的稳定抗性.中国水稻科学, 2008,22 (5):459464
\[5\]Huang J, Zhi M, Hai L, et al. Agrobacterium tumefaciensmediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer. Cell Res, 2001, 11(2):149155.
\[6\]顾红雅, 瞿礼佳, 明小天, 等. 植物基因与分子操作.北京: 北京大学出版社, 1995.
\[7\]方中达.植病研究方法.3版.北京: 中国农业出版社, 1996.
\[8\]Leevers S J, Vanhaesebroeck B, Waterfield M D. Signalling through phosphoinositide 3kinases: The lipids take centre stage. Curr Opin Cell Biol,1999, 11(2): 219225.
\[9\]amaj J, Read N D, Volkmann D, et al The endocytic network in plants. Trends Cell Biol，2005，15(8): 425433.
\[10\]Martinoia E, Maeshima M, Neuhaus H E. Vacuolar transporters and their essential role in plant metabolism. J Exp Bot, 2007, 58(1): 83102.
