Research Papers

Association Analysis of Rice Cold Tolerance at Tillering Stage with SSR Markers in japonica Cultivars in Northeast China

Expand
  • College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 

Received date: 2011-05-20

  Revised date: 2011-10-24

  Online published: 2012-07-10

Abstract

The cold tolerance of rice at the tillering stage was evaluated with 140 rice cultivars(lines) from Northeast China as materials and association analysis was conducted among 96 selected materials which differed in cold tolerance with 84 SSR markers. The experimental results showed: there were 20.0% of rice materials with better cold tolerance, 30.71% with intermediate cold tolerance and 49.28% with worse cold tolerance; Correlation analysis showed that rice cold tolerance at the tillering stage was significantly positively correlated with seed setting rate and single panicle weight. Rice cold tolerance at the tillering stage and growth duration were significantly negatively correlated; Eighteen markers associated with rice cold tolerance were detected through the association analysis, markers like RM214,RM445,RM506,RM106,RM215,RM182,RM270 and RM229 were mutual authentication with familybased linkage mapping. These markers can be used for selection of rice cold tolerance resources in Northeast China. Finally, a serial of elite alleles, loci and their carrier materials were screened out by anglicizing these associated markers.

Cite this article

ZHANG Yanmei, ZOU Detang* . Association Analysis of Rice Cold Tolerance at Tillering Stage with SSR Markers in japonica Cultivars in Northeast China[J]. Chinese Journal OF Rice Science, 2012 , 26(4) : 423 -430 . DOI: 10.3969/j.issn.10017216.2012.04.006

References

\[1\]郭晓丽, 王立刚,  邱建军, 等.基于GIS的东北地区水稻低温冷害区划研究. 江西农业大学学报,  2009,  31(3): 494498.

\[2\]崔锦, 李辑, 张爱忠, 等.东北夏季低温的研究进展.气象,  2007,  33(4): 39.

\[3\]姚佩珍.近四十年东北夏季低温冷害的气候特征.灾害学,  1995,  10(1): 5256.

\[4\]Baruah A R,  IshigoOka N,  Adachi M,  et al. Cold tolerance at the early growth stage in wild and cultivated rice. Euphytica,  2009, 165(3): 459470.

\[5\]Zhou L,  Zeng Y W,  Zheng W W, et al. Fine mapping a QTL qCTB7for cold tolerance at the booting stage on rice chromosome 7 using a nearisogenic line. Theor Appl Genet,  2010,  121(5): 895905.

\[6\]Suh  J P,  Jeung J U,  Lee  J I , et al. Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in coldtolerant genotypes of rice (Oryza sativa L.). Theor Appl Genet,  2010, 120(5): 985995.

\[7\]Saito K,  HayanoSaito Y,  Kuroki M,  et al. Mapbased cloning of the rice cold tolerance gene Ctb1. Plant Sci, 2010,  179(112): 97102.

\[8\]韩龙植,  乔永利,  曹桂兰,  等.水稻生长早期耐冷性QTL分析. 中国水稻科学, 2005, 19(2): 124125.

\[9\]Zhu Y L,  Song Q J, Hyten D L,  et al. Single nucleotide polymorphisms in soybean. Genetics,  2003,  163(3): 11231134.

\[10\]王荣焕,  王天宇,  黎裕. 关联分析在作物种质资源分子评价中的应用. 植物遗传资源学报,  2007,  8(3): 366372. 

\[11\]何静, 王文泉, 郑永清, 等.木薯栽培品种农艺性状与分子标记的关联分析. 热带作物学报,  2010, 31(5): 693698.

\[12\] Jin L, Bao J S. Progress on the traitmarker association analysis in plants. Mol Plant Breed,  2009,  7(6): 10481063

\[13\]Wen W, Mei H,Feng F,et al. Population structure and association mapping on chromosome 7 using a diverse panel of Chinese germplasm of rice (Oryza sativa L.). Theor Appl Genet, 2009, 119(3): 459470.

\[14\] Iwata H, Uga Y,  Yoshioka Y,  et al. Bayesian association mapping of multiple quantitative trait loci and its application to the analysis of genetic variation among Oryza sativa  L. germplasm. Theor Appl Genet,  2007,  114(8): 14371449.

\[15\]Eizenga G C,  Agrama H A,  Lee F N,  et al. Identifying novel resistance genes in newly introduced blast resistant rice germplasm. Crop Sci,  2006,  46(5): 18701878.

\[16\]Agrama H A,  Eizenga G C,  Yan W. Association mapping of yield and its components in rice cultivars. Mol Breed,  2007,  19(4): 341356.

\[17\]Huang X H,  Wei XH,  Sang T,  et al.  Genomewide association studies of 14 agronomic traits in rice landraces. Genetics 2010, 42(11): 961970.

\[18\]韩龙植,  张三元.水稻耐冷性鉴定评价方法. 植物遗传资源学报,  2004,  5(1): 7580.

\[19\] Pritchard J K,  Stephens M,  Donnelly P. Inference of population structure usingmultilocus genotype data. Genetics,  2000,  155: 945959.

\[20\] Edward Buckler Lab. Maize Diversity Research \[20070130\]http://www.maizegenetics.net/ bioinformatics.

\[21\]Breseghello F,  Mark E S. Association mapping of kernel size and milling quality in wheat (Triticum aestivum L) cultivars. Genetics, 2006, 172: 11651177.

\[22\]韩龙植,  乔永利,  张媛媛,  等.水稻孕穗期耐冷性QTLs分析. 作物学报, 2005,  31(5): 653657.

\[23\]赵国珍,  Yang S J, Yea J D,等.冷水胁迫对云南粳稻育成品种农艺性状的影响. 云南农业大学学报, 2010, (25)3:159163.

\[24\]Tenaillon M,  Sawkins M C,  Long A D,  et al. Pattern of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp.mays L.). Proc Natl Acad Sci USA, 2001,  98(16): 96619666.

\[25\] Zhu Y L,  Song Q J, Hyten D,  et al. Singlenucleotide polymorphisms in soybean. Genetics, 2003,  163(3): 11231133.

\[26\]Kraakman A T W,  Niks R E,  van den Berg P M M M, et al. Linkage disequilibrium mapping of yield and yield stability in modern spring barley cultivars. Genetics,  2004,  168(11): 436446.

\[27\]Garris A J,  McCouch S R,  Kresovich S K. Population structure and its effect on haplotype diversity and linkage disequilibrium surrounding the xa5 locus of rice (Oryza sativa.). Genetics,  2003, 165(2): 760768.

\[28\] McNally K L, Childs K L,Bohnert R,et al. Genomewide SNP variation reveals relationships among landraces and modern varieties of rice. Proc Natl Acad Sci USA,  2009, 106(30): 1227312278.

\[29\] Mather K A. The extent of linkage disequilibrium in rice (Oryza sativa L.). Genetics,  2007, 177(4): 22232232.

\[30\]杨小红,  严建兵,  郑艳萍,等. 植物数量性状关联分析研究进展. 作物学报,  2007,  33(4): 523530.

\[31\]乔永利,韩龙植,安永平,等.水稻芽期耐冷性QTL的分子定位. 中国农业科学,  2005,  38(2): 217221.

\[32\] 吴杏春,  王茵,  林文雄.水稻苗期耐冷性状的QTL分析. 中国生态农业学报,  2008,   16(4): 10671069.

\[33\] Lou Q J,  Chen L,   Sun Z X,  el at. A major QTL associated with cold tolerance at seedling stage in rice (Oryza sativa L). Euphytica,  2007,  158(1/2): 8794.

\[34\]赵正武.水稻越冬耐冷性遗传变异及QTL定位研究\[D\]. 成都:四川农业大学,2006.

\[35\]夏瑞祥.东乡野生稻苗期耐冷性、株高和穗长的QTL分析\[D\].  扬州:扬州大学,2008.

\[36\] Andaya V C,   Mackill D J. QTLs conferring cold tolerance at the booting stage of rice using recombinant inbred lines from a japonica×indica cross. Thero Appl Genet,2003,106(6): 10841090.
Outlines

/

Tel: 0571-63370278 E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.