Short Communications

Identification of QTL for Protein Content in Brown and Milled Rice in Two Enironments

Expand
  • Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China;

Received date: 2011-09-13

  Revised date: 2011-10-19

  Online published: 2012-05-10

Abstract

Based on single seed descend method a recombinant inbred lines  population consisting of 190 lines  was created by crossing two rice varieties with similar apparent amylose content, Nipponbare (japonica) and 9311 (indica) by QTLs for brown rice protein content(BRPC) and milled rice protein content (MRPC) with a genetic linkage map including 202 SSR, CAP and STS markers were identified. By composite interval mapping method in two environments sites (Sanya Hainan Province, China and Hefei Anhui Province, China), a total of nine QTLs were identified, indicating that rice protein content was controlled by multiple genes.  qBRPC1.3 was detected at the two sites with high stability, explaining 9.4% of total variation. The interval RM578-RM10596 on chromosome 1, controlled BRPC and MRPC, explaining 6.4% and 5.7% of total variation, respectively. 

Cite this article

YANG Yachun, NI Dahu, SONG Fengshun, LI Li, FENG Guang, LI Zefu, YANG Jianbo* . Identification of QTL for Protein Content in Brown and Milled Rice in Two Enironments[J]. Chinese Journal OF Rice Science, 2012 , 26(3) : 351 -355 . DOI: 10.3969/j.issn.10017216.2012.03.014

References

\[1\]Tan Y F, Sun M, Xing Y Z,  et al.Mapping quantitative trait loci for milling quality protein content and color characteristics of rice using a recombinant inbred line population derived from an elite rice hybrid.Theor Appl Genet,  2001, 103(67): 10371045.

\[2\]于永红, 朱智伟, 樊叶杨, 等.应用重组自交系群体检测控制水稻糙米粗蛋白和粗脂肪含量的QTL.作物学报, 2006, 32(11): 17121716.

\[3\]张涛, 郑家奎, 吴先军, 等.水稻糙米蛋白质含量的QTL定位.分子植物育种, 2009, 7(1): 6772.

\[4\]Rogers S O, Bendch A J. Extraction of DNA from plant tissues. Plant Mol Biol Man,  1988, 6: 110.

\[5\]杨亚春, 倪大虎, 宋丰顺, 等. 不同生态地点下稻米外观品质性状的OTL定位分析. 中国水稻科学, 2011, 25(1): 4351.

\[6\]Mc Couch S R. Gene nomenclature system for rice. Rice,  2008, 1(1): 7284.

\[7\]王熙军. 稻米蛋白质含量与气象条件的关系探讨.广西气象, 1997, 18(4): 5153.

\[8\]周广洽, 徐孟亮, 谭周, 等.温光对稻米蛋白质及氨基酸含量的影响.生态学报, 1997, 17(5): 537542.

\[9\]程方民, 钟连进.不同气候生态条件下稻米品质性状的变异及主要影响因子分析.中国水稻科学, 2001,  15(3): 187191.

\[10\]McCouch S R, Teytelman L, Xu Y B, et al. Development and mapping of 2240 new SSR markers for rice ( Oryza sativa L.). DNA Res,  2002, 9: 199207.

\[11\]Causse M A, Fulton T M, Cho Y G, et al. Saturated molecular map of the rice genome based on an inter specific backcross population. Genetics,  1994, 138: 12511274.

\[12\]Hu Z L, Li P, Zhou M Q,  et al.Mapping of quantitative trait loci (QTLs) for rice protein and fat content using doubled haploid lines.Euphytica,  2004, 135(1): 4754.

\[13\]李晨, 潘大建, 孙传清, 等.水稻糙米高蛋白基因的QTL定位.植物遗传资源学报, 2006, 7(2): 170174.

\[14\]钟明, 王令强, 罗利军, 等.利用RIL群体比较定位糙米和精米蛋白质含量的QTL.分子植物育种, 2007, 5(5): 631638.

\[15\]翁建峰, 万向元, 吴秀菊, 等.利用CSSL群体研究稻米AC和PC相关QTL表达稳定性.作物学报, 2006,  32(1): 1419.

\[16\]高振宇, 曾大力, 崔霞, 等.水稻稻米糊化温度控制基因ALK的图位克隆及其序列分析.中国科学: C辑, 2003, 33(6): 481487.

\[17\]Ryoo N,  Yu C,  Park C S,  et al.Knockout of a starch synthase gene OsSSIIIa/Flo5  causes whitecore floury endosperm in rice (Oryza sativa L.). Plant Cell Rep,  2007, 26(7):10831095.

\[18\]Wan X Y, Wan J M, Weng J F,  et al.Stability of QTLs for rice grain dimension and endosperm chalkiness characteristics across eight environments. Theor Appl Genet,  2005, 110(7): 13341346.

\[19\]Xiao J, Li J, Yuan L,  et al.Identification of QTLs affecting traits of agronomic importance in a recombinant inbred population derived from a subspecific rice cross.Theor Appl Genet,  1996, 92: 230244.

\[20\]Hittalmani S, Huang N, Courtois B,  et al.Identification of QTL for growth and grain yieldrelated traits in rice acrossnine locations of Asia. Theor Appl Genet,  2003, 107: 679690.

\[21\]郭益全, 刘清, 张德梅, 等.籼稻烹调与食用品质及谷粒性状之遗传.中华农业研究, 1985, 34(3):243257.

\[22\]Hussain A A, Maurya D M, Vaish C P. Study on quality status of indigenous upland rice(Oryza sativa L.).Indian J Genet &  Plant Breeding,  1987, 47(2): 145152.

\[23\]汤圣祥.我国杂交水稻蒸煮与食味品质的研究.中国农业科学, 1987, 20(5): 1722.

\[24\]Weber H, Rolletschek H, Heim U. Antisense inhibition of ADPglucose pyrophosphorylase in developing seeds of Vicia narbonensis  moderately decreases starch but increases protein content and affects seed maturation. Plant J,  2000, 24(1): 3343.

\[25\]Kavakli H I, Slattery C J, Ito H. The conversion of carbon and nitrogen into starch and storage proteins in developing storage organs: An overview. Aust J Plant Physiol,  2000, 27: 561570.

\[26\]Yanagisawa S, Akiyama A, Kisaka H.Metabolic engineering with Dof1 transcription factor in plants: Improved nitrogen assimilation and growth under lownitrogen conditions. Proc Natl Acad Sci USA,  2004, 101: 78337838.
Outlines

/

Tel: 0571-63370278 E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.