Genetic Analysis and Gene Mapping of a Light Brown Spotted Leaf Mutant in Rice

FENG Baohua1, YANG Yang1,2, SHI Yongfeng1, LIN Lu3, CHEN Jie1, HUANG Qina1， WEI Yanlin1,2, Hei LEUNG4, WU Jianli1,*

doi:10.3969/j.issn.10017216.2012.03.007

Chinese Journal OF Rice Science >

2012 , Vol. 26 >Issue 3: 297 - 301

DOI: https://doi.org/10.3969/j.issn.10017216.2012.03.007

Research Papers

Genetic Analysis and Gene Mapping of a Light Brown Spotted Leaf Mutant in Rice

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¹ State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China; ² College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; ³ College of Life Sciences, Anhui Normal University, Wuhu 241000， China; ⁴ International Rice Research Institute, DAPO Box 7777 Metro Manila, Philippines;

Received date: 2011-08-11

Revised date: 2011-10-19

Online published: 2012-05-10

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Abstract

A light brown spottedleaf mutant of rice was isolated from an EMSinduced IR64 mutant bank. The mutant, designated as lbsl1（light brown spottedleaf 1）, displayed light brown spot in the whole growth period from the first leaf to the flag leaf under natural summer field conditions. Agronomic traits including plant height, growth duration, seedsetting rate and 1000grain weight of the mutant were significantly affected. Genetic analysis showed that the mutation was controlled by a single recessive gene, tentatively termed lbsl1(t), which was mapped to the short arm of chromosome 6. With developed SSR markers, the gene was finally delimited to an interval of 130 kb between markers RM586 and RM588. The lbsl1(t) gene is likely a novel rice spottedleaf gene since no other similar genes have been identified near the chromosomal region. The genetic data and recombination populations provided will facilitate further fine mapping and cloning of the gene.

Key words： rice; leaf color; spottedleaf mutant; molecular marker; gene mapping

Cite this article

FENG Baohua1, YANG Yang1,2, SHI Yongfeng1, LIN Lu3, CHEN Jie1, HUANG Qina1， WEI Yanlin1,2, Hei LEUNG4, WU Jianli1,* . Genetic Analysis and Gene Mapping of a Light Brown Spotted Leaf Mutant in Rice[J]. Chinese Journal OF Rice Science, 2012 , 26(3) : 297 -301 . DOI: 10.3969/j.issn.10017216.2012.03.007

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