The effect of drywet alternate water treatment during grain filling on grain quality and its physiological mechanisms were investigated with Yangdao 6 (indica) and Wuyujing 3 (japonica) as materials. The results showed milling quality and appearance quality were improved, breakdown values of rice flour were increased and setback values were decreased under alternate moderate drying and wetting treatment (MD). The results were reversed under alternate severe drying and wetting treatment (SD). The activities of sucrose synthase (SuSase), ADP glucose pyrophosphorylase (ADPGase), soluble starch synthase (SSSase), and Qenzyme in grains increased under MD during grain filling, while decreased under SD. The enzyme activities were significantly correlated with main indices of grain quality. The concentrations of 1aminocylopropane1carboxylic acid (ACC,biosynthetical precursor of ethylene) in root bleeding, the concentration of ACC and ethylene evolution rate in grains were significantly decreased under MD, while significantly increased under SD. The concentrations of ACC in root bleeding, the concentration of ACC and ethylene evolution rate in grains were significantly or extremely significantly and negatively correlated with the activities of SuSase, ADPGase, SSSase and Qenzyme. The shifting concentration of ACC in root under alternate dryingwetting treatment during grain filling affected the concentration of ACC and ethylene evolution rate in grains, which influenced the activities of enzymes involved in starch synthesis, and grain quality finally.
LIU Lijun1, LI Hongwei1, ZHAO Buhong2, WANG Zhiqin1, YANG Jianchang1，*
. Effects of Alternate Dryingwetting Irrigation During Grain Filling on Grain Quality and Its Physiological Mechanisms in Rice [J]. Chinese Journal OF Rice Science, 2012
, 26(1)
: 77
-84
.
DOI: 10.3969/j.issn.10017216.2012.01.013
\[1\]颜龙安, 李季能, 钟海明. 优质稻米生产技术. 北京: 中国农业出版社, 1999: 3037.
\[2\]郑家国, 任光俊, 陆贤军, 等. 花后水分亏缺对水稻产量和品质的影响. 中国水稻科学, 2003, 17(3): 239243.
\[3\]王人民, 丁元树. 水稻抽穗和结实期的生态因子研究: Ⅰ. 土壤水分对早稻结实和籽粒品质的影响. 浙江农业大学学报:农业与生命科学版, 1989, 15(1): 1420.
\[4\]邓定武, 谭正之, 龙兴汉. 灌溉对杂交水稻产量及稻米品质的影响. 作物研究, 1990, 4(2): 79.
\[5\]Gomex K A. Effect of environment on protein and amylose content of rice//Proceedings of the Workshop of Chemical Aspects of Rice Grain Quality. Philippines: International Rice Research Institute, 1979: 5968.
\[6\]蔡一霞, 朱庆森, 王志琴, 等. 结实期土壤水分对稻米品质的影响. 作物学报, 2002, 28(5): 601608.
\[7\]蔡一霞, 王维, 张祖建, 等. 水旱种植下多个品种蒸煮品质和稻米RVA谱的比较性研究. 作物学报, 2003, 29(4): 508613.
\[8\]杨建昌, 袁莉民, 唐成, 等. 结实期干湿交灌溉对稻米品质及籽粒中一些酶活性的影响. 作物学报, 2005, 31（8）: 10521057.
\[9\]Murata Y, Matsushima S. Rice//Evans L T. Crop Physiology.London, UK: Cambridge University Press, 1975: 7599.
\[10\] Yoshida S. Physiological aspects of grain yield.Ann Rev Plant Physiol, 1972, 23: 437464.
\[11\]Nakamura Y, Yuki K, Park S Y. Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiol, 1989, 30: 833839.
\[12\] Nakamura Y, Yuki K. Changes in enzyme activities associated with carbohydrate metabolism during development of rice endosperm.Plant Sci, 1992, 82: 1520.
\[13\] Kato T.Change of sucrose synthase activity in developing endosperm of rice cultivars. Crop Sci, 1995, 35: 827831.
\[14\] Wang F, Sanz A, Brenner M L,et al. Sucrose synthase, starch accumulation, and tomato fruit sink strength. Plant Physiol, 1993, 101: 321327.
\[15\] Martin C, Smith A M. Starch biosynthesis.Plant Cell, 1995, 7: 971985.
\[16\]Davies P J. Introduction//Davies P J. Plant Hormones, Biosynthesis, Signal Transduction, Action! Dordrecht, The Netherlands: Kluwer Academic Publishers, 2004: 135.
\[17\]del Pozo J C, LopezMatas M A, RamirezParra E, et al. Hormonal control of the plant cell cycle. Physiol Plant, 2005, 123: 173183.
\[18\]Morgan P W, Drew M C. Ethylene and plant responses to stress. Physiol Plant, 1997, 100: 620630.
\[19\]Yang J C, Zhang J H, Wang Z Q, et al. Activities of enzymes involved in sucrosetostarch metabolism in rice grains subjected to water stress during filling. Field Crops Res, 2003, 81: 6981.
\[20\]赵步洪, 张文杰, 常二华, 等. 水稻灌浆期籽粒中淀粉合成关键酶的活性变化及其与灌浆速率和蒸煮品质的关系.中国农业科学, 2004, 37（8）: 11231129.
\[21\]Beltrano J, Carbone A, Montaldi E R, et al. Ethylene as promoter of wheat grain maturation and ear senescence. Plant Growth Regul, 1994, 15: 107117.
\[22\]Cheng C Y, Lur H S. Ethylene may be involved in abortion of the maize caryopsis. Physiol Plant, 1996, 98: 245252.
\[23\]刘凯, 叶玉秀, 唐成, 等. 水稻籽粒中乙烯和ACC对土壤水分的反应及其与籽粒灌浆的关系. 作物学报, 2007, 33(4): 539546.
\[24\]Zhang H, Chen T T, Wang Z Q, et al. Involvement of cytokinins in the grain filling of rice under alternate wetting and drying irrigation. J Exp Bot, 2010, 61: 37193733.
\[25\] Liang J S, Zhang J H, Cao X Z. Grain sink strength may be related to the poor grain filling of indicajaponica rice (Oryza sativa) hybrids. Physiol Plant, 2001, 112: 470477.
\[26\] 杨建昌, 彭少兵, 顾世梁, 等. 水稻灌浆期籽粒中3个与淀粉合成有关的酶活性变化.作物学报, 2001, 27（2）: 157164.
