Fine Mapping of a Major QTL qHd8.1 for Heading Date in Rice

ZHOU Yong, CUI Guokun, ZHANG Yanzhou, GUAN Chengran, CHANG Siyuan, GU Minghong, LIANG Guohua*

doi:10.3969/j.issn.10017216.2012.01.008

Chinese Journal OF Rice Science >

2012 , Vol. 26 >Issue 1: 43 - 48

DOI: https://doi.org/10.3969/j.issn.10017216.2012.01.008

Research Papers

Fine Mapping of a Major QTL qHd8.1 for Heading Date in Rice

Expand

Jiangsu Key Laboratory for Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China;

Received date: 2011-01-08

Revised date: 2011-02-19

Online published: 2012-01-10

Fold

Abstract

C23011, containing a single introgressed segment of chromosome 8, was derived from the CSSL population with Nipponbare as the donor and Guanglu′ai 4 as the recurrent parent. In normal longday conditions, its heading date was 14.1 d shorter than that of Nipponbare. Under shortday conditions, however, no obvious difference in heading date between C23011 and Nipponbare was observed. A segregation population derived from a BC4F2 individual with the heterozygous target segment was used for genetic analysis and gene mapping. The ratio of late heading plants to early heading plants showed a 3∶1 frequency distribution，suggesting that the heading date variation was controlled by a single major QTL, which was named qHd8.1. A physical map was constructed encompassing the qHd8.1 locus and the locus was delimited to a 59.94 kb interval between markers S8111 and S849 on BAC clone AP005164 using 1628 individuals with shorter heading date originated from the BC4F3 populations. There are seven predicted genes within this region.

Key words： rice; heading date; quantitative trait loci; fine mapping

Cite this article

ZHOU Yong, CUI Guokun, ZHANG Yanzhou, GUAN Chengran, CHANG Siyuan, GU Minghong, LIANG Guohua* . Fine Mapping of a Major QTL qHd8.1 for Heading Date in Rice[J]. Chinese Journal OF Rice Science, 2012 , 26(1) : 43 -48 . DOI: 10.3969/j.issn.10017216.2012.01.008

References

\[1\]钱前. 水稻基因设计育种. 北京: 科学出版社, 2007.

\[2\]林鸿宣, 钱惠荣, 熊振民, 等. 几个水稻抽穗期主效基因与微效基因的定位研究. 遗传学报, 1996, 23: 205213.

\[3\]Yano M, Harushima Y, Nagamura Y, et al. Identification of quantitative trait loci controlling heading date in rice using a highdensity linkage map. Theor Appl Genet, 1997, 95: 10251032.

\[4\]Yano M, Katayose Y, Ashikari M, et al. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONTANS. Plant Cell, 2000, 12: 24732484.

\[5\]Yano M, Kojima S, Takahashi Y, Lin H X, et al. Genetic control of flowering time in rice, a shortday plant. Plant Physiol, 2001, 127: 14251429.

\[6\]Yamamoto T, Kuboki Y, Lin S Y, et al. Fine mapping of quantitative trait loci Hd1, Hd2, and Hd3, controlling heading date of rice, as single Mendelian factors. Theor Appl Genet, 1998, 97: 3744.

\[7\]Lin H X, Ashikari M, Yamanouchi U, et al. Identication and characterization of a quantitative trait locus, Hd9, controlling heading date in rice. Breed Sci, 2002, 52: 3541.

\[8\]Matsubara K, Kono I, Hori K, et al. Novel QTLs for photoperiodic flowering revealed by using reciprocal backcross inbred lines from crosses between japonica rice cultivars. Theor Appl Genet, 2008, 117: 935945.

\[9\]Takahashi Y, Shomura A, Sasaki T, et al. Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2. Proc Natl Acad Sci USA, 2001, 98: 79227927.

\[10\]Xue W Y, Xing Y Z, Weng X Y, et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet, 2008, 40: 761767.

\[11\]Doi K, Izawa T, Fuse T, et al. Ehd1， a Btype response regulator in rice, confers shortday promotion of flowering and controls FTlike gene expression independently of Hd1. Genes Dev, 2004, 18: 926936.

\[12\]Kojima S, Takahashi Y, Kobayashi Y, et al. Hd3a， a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under shortday conditions. Plant Cell Physiol, 2002, 43: 10961105.

\[13\]Matsubara K, Yamanouchi U, Wang Z X, et al. Ehd2, a rice ortholog of the maize INDETERMINATE1 gene, promotes flowering by upregulating Ehd1. Plant Physiol, 2008, 148: 14251435.

\[14\]Kim S L, Lee S, Kim H J, et al. OsMADS51 is a shortday flowering promoter that functions upstream of Ehd1, OsMADS14, and Hd3a. Plant Physiol, 2007, 145: 14841494.

\[15\]Wu C, You C, Li C, et al. RID1, encoding a Cys2/His2type zinc finger transcription factor, acts as a master switch from vegetative to floral development in rice. Proc Natl Acad Sci USA, 2008, 105: 1291512920.

\[16\]Lee Y S, Jeong D H, Lee D Y, et al. OsCOL4 is a constitutive flowering repressor upstream of Ehd1 and downstream of OsphyB. Plant J, 2010, 63: 1830.

\[17\]Park S J, Kim S L, Lee S, et al. Rice Indeterminate 1 (OsId1) is necessary for the expression of Ehd1 (Early heading date 1） regardless of photoperiod. Plant J, 2008, 56: 10181029.

\[18\]Hayama R, Yokoi S, Tamaki S, et al. Adaptation of photoperiodic control pathways produces shortday flowering in rice. Nature, 2003, 422: 719722.

\[19\]姜树坤, 徐正进, 陈温福. 水稻QTL 图位克隆的特征分析. 遗传, 2008, 30(9): 11211126.

\[20\]杨德卫, 张亚东, 朱镇, 等. 基于CSSL的水稻抽穗期QTL定位及遗传分析. 植物学报, 2010, 45(1): 189197.

\[21\]何风华, 席章营, 曾瑞珍, 等. 利用单片段代换系定位水稻抽穗期QTL. 中国农业科学, 2005, 38(8): 15051513.

\[22\]赵芳明, 张桂权, 曾瑞珍, 等. 用单片段代换系(SSSLs)研究水稻株高及其构成因素QTL加性及上位性效应. 作物学报, 2009, 35(1): 4856.

\[23\]赵芳明, 刘桂富, 朱海涛, 等. 用单片段代换系对不同时期水稻分蘖数QTL的非条件和条件定位. 中国农业科学, 2008, 41(2): 322330.

\[24\]王雨, 孙永建, 陈灯银, 等. 水稻染色体片段代换系对氮、磷胁迫反应差异及其QTL分析. 作物学报, 2009, 35(4): 580587.

\[25\]朱文银, 杨德卫, 林静, 等. 利用染色体片段置换系定位水稻落粒性主效QTL. 植物学通报, 2008, 25(4): 443448.

\[26\]刘冠明, 李文涛, 曾瑞珍, 等. 水稻亚种间单片段代换系的建立. 中国水稻科学, 2003, 17(3): 201204.

\[27\]Yang D W, Zhu Z, Zhang Y D, et al. Substitution mapping of QTL for panicle exertion using CSSL in rice (Oryza sativa L.). Hereditas, 2009, 31(7): 741747.

\[28\]Liu G F, Zeng R Z, Zhu H T, et al. Dynamic expression of nine QTLs for tiller number detected with single segment substitution lines in rice. Theor Appl Genet, 2009, 118: 443453.

\[29\]Ando T, Yamamoto T, Shimizu T, et al. Genetic dissection and pyramiding of quantitative traits for panicle architecture by using chromosomal segment substitution lines in rice. Theor Appl Genet, 2008, 116: 881890.

\[30\]Shan J X, Zhu M Z, Shi M, et al. Fine mapping and candidate gene analysis of spd6, responsible for small panicle and dwarfness in wild rice (Oryza rufipogon Griff.). Theor Appl Genet, 2009, 119: 827836.

\[31\]Takai T, Ohsumi A, Sanoh Y, et al. Detection of a quantitative trait locus controlling carbon isotope discrimination and its contribution to stomatal conductance in japonica rice. Theor Appl Genet, 2009, 118: 14011410.

\[32\]邢永忠, 徐才国, 华金平, 等. 水稻株高和抽穗期基因的定位和分离. 植物学报, 2001, 43(8): 721726.

\[33\]Lin H, Yamamoto T, Sasaki T, et al. Characterization and detection of epistatic interactions of 3 QTLs, Hd1, Hd2, and Hd3, controlling heading date in rice using nearly isogenic lines. Theor Appl Genet, 2000, 101: 10211028.

\[34\]Li Z, Pinson S, Stansel J, et al. Identification of quantitative trait loci (QTL) for heading date and plant height in rice using RFLP markers. Theor Appl Genet, 1995, 91: 374381.

\[35\]Zhou Y, Li W, Wu W, et al. Genetic dissection of heading time and its components in rice. Theor Appl Genet, 2001, 102: 12361242.

\[36\]Nonoue Y, Fujino K, Hirayama Y, et al. Detection of quantitative trait loci controlling extremely early heading in rice. Theor Appl Genet, 2008, 116: 715722.

\[37\]Zhang Y S, Luo L J, Xu C G, et al. Quantitative trait loci for panicle size, heading date and plant height cosegregating in traitperformance derived nearisogenic lines of rice (Oryza sativa). Theor Appl Genet, 2006, 113: 361368.

\[38\]Lin H, Liang Z, Sasaki T, et al. Fine mapping and characterization of quantitative trait loci Hd4 and Hd5 controlling heading date in rice. Breed Sci, 2003, 53: 5159.

\[39\]Wei X J, Xu J F, Guo H N. et al. DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously. Plant Physiol, 2010, 153: 17471758.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References