The forward and reverse subtracted cDNA libraries of imidaclopridresistant and imidaclopridsusceptible brown planthopper (Nilaparvata lugens Stl) populations were constructed by suppression subtractive hybridization (SSH), and the relative genes regulated by imidacloprid were cloned. The results showed that 120 positive clones obtained represented 92 different single genes, in which 17 shared higher homology with known protein genes of animals in the GenBank. These single genes involved signal transduction, biotic and abiotic stress, reproduction, and etc.
ZHANG Juefeng, HE Yueping, CHEN Jianming*, CHEN Liezhong
. Construction of Suppression Subtractive Hybridization cDNA Library of Imidaclopridresistant and Imidaclopridsusceptible Brown Planthopper[J]. Chinese Journal OF Rice Science, 2012
, 26(1)
: 21
-26
.
DOI: 10.3969/j.issn.10017216.2012.01.005
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