To demonstrate the biological function of OsBTF3 gene in rice resistance or tolerance to salt and cold stress, the gene transcription of OsBTF3 responsive to salt and cold treatments was quantified via realtime PCR assay, and resistance to the stress of transgenic rice seedlings with overexpressed and RNAisilenced OsBTF3 was analyzed. The results showed that gene expression of OsBTF3 was significantly decreased in rice plants under salt stress, while lightly reduced under cold stress. The resistance to salt and cold stress was enhanced highly in the seedlings of transgenic lines with overexpressed OsBTF3, while decreased in the RNAisilenced OsBTF3 lines. The results revealed that OsBTF3 might function as a key regulator for abiotic stress resistance in rice.
LI Guangxu1, 2, CHEN Huamin1, WU Maosen1, HE Chenyang1,*
. Resistance to High Salt and Cold Stress of Transgenic Rice Seedlings with Overexpressed and RNAisilenced OsBTF3[J]. Chinese Journal OF Rice Science, 2012
, 26(1)
: 5
-8
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DOI: 10.3969/j.issn.10017216.2012.01.002
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