A rice mutant with significantly shorter root hairs was isolated from an EMSinduced mutant library of indica Kasalath. The mutant was named as Kasalath short root hair 1 (ksrh1). Except shorter root hairs, there was no significant difference between ksrh1 and its wild type. Genetic analysis showed that the mutant was controlled by a single recessive nuclear gene. To map the gene KSRH1, the mutant ksrh1 and Nipponbare were crossed to generate an F2 population. By using the published SSR markers and some newly designed STS markers, KSRH1 was mapped to a 67 kb region between the STS markers S3578 and S3584 on the long arm of chromosome 1.
DING Wona, WU Jing, LUO Lili, QIAN Zhouting, ZHU Shihua
. Genetic Analysis and Gene Mapping of a Short Root Hair Mutant ksrh1 in Rice[J]. Chinese Journal OF Rice Science, 2012
, 26(1)
: 1
-4
.
DOI: 10.3969/j.issn.10017216.2012.01.001
\[1\]Gilroy S, Jones D L. Through form to function: Root hair development and nutrient uptake. Trends Plant Sci, 2000, 5(2): 5660.
\[2\]Cho H T, Cosgrove D J. Regulation of root hair initiation and expansin gene expression in Arabidopsis. Plant Cell, 2002, 14(12): 32373253.
\[3\]Horn R, Yi K, Menand B, et al. Root epidermal development in Arabidopsis. Annu Plant Rev, 2009, 37: 6482.
\[4\]Libault M, Brechenmacher L, Cheng J, et al. Root hair systems biology. Trends Plant Sci, 2010, 15: 641650.
\[5\]Ding W N, Yu Z M, Tong Y L, et al. A transcription factor with a bHLH domain regulates root hair development in rice. Cell Res, 2009, 19: 13091311.
\[6\]Kim C M, Park S H, Je B I, et al. OsCSLD1, a cellulose synthaselike D1 gene, is required for root hair morphogenesis in rice. Plant Physiol, 2007, 143(3): 12201230.
\[7\]Won S K, Kumari S, Choi S B, et al. Root hairspecific EXPANSIN B genes have been selected for Graminaceae root hairs. Mol Cell, 2010, 30(4): 369376.
\[8\]Yu Z M, Kang B, He X W, et al. Root hairspecific EXPANSINs modulate root hair elongation in rice. Plant J, 66(5):725734.
\[9\]You T, Toyota M, Ichii M, et al. Molecular cloning of a root hairless gene rth1 in rice. Breeding Sci, 2009, 59(1): 1320.
\[10\]Yoshida S, Forno D A, Cock J H, et al. Laboratory Manual for Physiological Studies of Rice. 3rd ed. Manila, the Philippines: the International Rice Research Institute, 1976.
\[11\]张向前, 邹金松, 朱海涛, 等. 水稻早熟多子房突变体fon5的遗传分析和基因定位. 遗传, 2008, 30: 13491355.
\[12\]Michelmore R W, Papan I, Kesseli R V. Identification of markers linked to disease resistance genes by bulked segregantanalysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 98289832.
\[13\] Vance C P, UhdeStone C, Allan D L. Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource. New Phytol, 2003, 157(3): 423447.