With 16 hybrid rice male sterile lines, restorer lines and combinations as materials, a capillary electrophoresis detection method with fluorescent SSR markers in rice was primarily developed. The accurate size of DNA fragment could be detected by the capillary electrophoresis detection method with fluorescent SSR markers. The capillary electrophoresis detection has the advantages of data accuracy and efficiency compared with polyacrylamide gel electrophoresis detection. It provides a feasible and reliable method for rice DNA fingerprint identification.
CHENG Benyi, XIA Junhui, GONG Junyi, YANG Shihua*
. Application of Capillary Electrophoresis Detection with Fluorescent SSR Markers in Rice DNA Fingerprint Identification[J]. Chinese Journal OF Rice Science, 2011
, 25(6)
: 672
-676
.
DOI: 10.3969/j.issn.10017216.2011.06.016
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