Effect of Fixation and Staining Methods on Observation of&nbsp; Endosperm Cell&nbsp;&nbsp; Structure in Rice

LIU  Zhi1, WANG  Lingling1, ZHOU  Weidong2, CHEN  Yifang2, WANG  Zhong1,*

doi:10.3969/j.issn.10017216.2011.06.015

Chinese Journal OF Rice Science >

2011 , Vol. 25 >Issue 6: 667 - 671

DOI: https://doi.org/10.3969/j.issn.10017216.2011.06.015

Experimental Techniques

Effect of Fixation and Staining Methods on Observation of Endosperm Cell Structure in Rice

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¹ Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Crop Physiology, Ecology and Cultivation in Middle and Lower Reaches of Yangtze River, Ministry of Agriculture, Yangzhou 225009, China;² Testing Center， Yangzhou University, Yangzhou 225009, China;

Received date: 2010-12-30

Revised date: 2011-03-11

Online published: 2011-11-10

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Abstract

The caryopses of rice cv. Yangdao 6 were harvested at 3 to 10 days after flowering and prepared for the conventional glutaraldehydeosmium tetroxide (GAOsO4) fixation and for the potassium permanganate (KMnO4) fixation, respectively. After being embedded with Spurr resin, the sections were made and stained by ammonium methylbenzene blue staining(AMB), periodic acid schiff(PAS), multicolor dye and restained by PASAMB, respectively. The different effects of the two fixation and four stained methods were observed and compared. The results revealed that better semithin sections could be obtained by GAOsO4 fixation. The ultrathin sections by GAOsO4 fixation presented a clearer cell structure, but the endomembrane structure was not clear enough. For the potassium permanganate fixation method, the endomembrane structure was clear, while polysaccharide and protein were in poor colour. It is recommended that selecting proper fixation and staining methods should be based on the observation of the contents in the experiments.

Key words： rice; endosperm cell; fixation method; staining method; semithin section; ultrathin section

Cite this article

LIU Zhi1, WANG Lingling1, ZHOU Weidong2, CHEN Yifang2, WANG Zhong1,* . Effect of Fixation and Staining Methods on Observation of Endosperm Cell Structure in Rice[J]. Chinese Journal OF Rice Science, 2011 , 25(6) : 667 -671 . DOI: 10.3969/j.issn.10017216.2011.06.015

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