An F2 population consisting of 176 individuals was developed from a cross between an indica maintainer line Zhenshan 97B and a japonica maintainer line Liao 91B and a genetic map containing 123 SSR markers was constructed based on the population. Pollen fertility and spikelet fertility were investigated in three seasons. Three maineffect (additive effect and dominant effect) quantitative trait loci (QTLs) for pollen fertility (qPF3, qPF5 and qPF6) and four maineffect QTLs for spikelet fertility (qSF3, qSF5, qSF6 and qSF8) were detected. Among them, qPF6 and qSF6, and qPF5 and qSF5, important QTLs for rice fertility, were mapped on the same locations. In addition, three pairs of QTL interaction for pollen fertility and four pairs for spikelet fertility were detected, suggesting that epistatic effects play important roles in the fertility of indicajaponica hybrids.
\[1\]杨守仁. 籼粳稻杂交育种的进展及前景. 中国农业科学, 1986(5): 1518.
\[2\]袁隆平. 选育水稻亚种间杂交组合的策略. 杂交水稻, 1996(2): 13.
\[3\]Ikehashi H, Araski H. Genetics of sterility in remote crosses of rice//Rice Genetics. Manila, Philippines: IRRI, 1986: 119130.
\[4\]Chen J J, Ding J H, Ouyang Y D, et al. A triallelic system of S5 is a major regulator of the reproductive barrier and compatibility of indicajaponica hybrids in rice. Proc Natl Acad Sci USA, 2008, 105(32): 1143611441.
\[5\]杨杰, 王军, 曹卿, 等. 水稻广亲和基因S5n 的功能标记开发及其应用. 作物学报, 2009, 35(11): 20002007.
\[6\]张桂权, 卢永根, 张华, 等. 栽培稻(Oryza sativa L.)杂种不育性的遗传研究: Ⅳ. F1花粉不育的基因型. 遗传学报, 1994, 21(1): 3441.
\[7\]苏菁, 刘耀光. 栽培稻(Oryza sativa L.)亚种间F1花粉不育基因Sa的精细定位及克隆. 分子植物育种, 2003, 1(5/6): 757758.
\[8\]李文涛, 曾瑞珍, 张泽民, 等. 水稻F1花粉不育基因座Sb的精细定位. 科学通报, 2006, 51(4): 404408.
\[9\]杨存义, 陈忠正, 庄楚雄, 等. 水稻籼粳杂种不育基因座Sc的遗传图和物理图精细定位. 科学通报, 2004, 49(13): 12731277.
\[10\]Kubo T, Yoshimura A. Linkage analysis of an F1 sterility gene in japonica/indica cross of rice.Rice Genet Newsl, 2001,18: 5253.
\[11\]Matsuzaki S Y, Yoshimura A. Mapping of a gene for pollen semisterility rice chromosome 8 of rice. Rice Genet Newsl, 2001,18: 5961.
\[12\]Matsuzaki S Y, Yoshimura A. Mapping of pollen semisterility gene，S28(t)，on rice chromosome 4. Rice Genet Newsl, 2002, 19: 8082.
\[13\]Zhu S S, Jian G L, Wang C M, et al. The origin of a weedy rice Ludao in China deduced by analysis of its hybrid sterility genes. Breeding Sci, 2005, 55(4): 409414.
\[14\]Zhu S S, Wang C M, Zheng T Q, et al. A new gene causing hybrid sterility located on chromosome 2 in a remote cross of rice. Plant Breeding, 2005, 124(5): 16.
\[15\]Zhao Z G, Wang C M, Jiang L, et al. Identification of a new hybrid sterility gene in rice (Oryza sativa L.). Euphytica, 2006, 151(3): 331337.
\[16\]Li D T, Chen L M, Jiang L, et al. Fine mapping of S32(t), a new gene causing hybrid embryo sac sterility in a Chinese landrace rice (Oryza sativa L.). Theor Appl Genet, 2007, 114(3): 515524.
\[17\]Jing W, Liu L L, Jiang L, et al. Mapping of S33(t) and S34(t) for pollen sterility in hybrids between a weedy strain and a japonica variety in rice. Rice Genet Newsl, 2007, 23: 1619.
\[18\]严长杰, 梁国华, 顾世梁, 等. 水稻籼粳杂种不育性及其类型. 作物学报, 2003, 29(4): 574580.
\[19\]McCouch S R, Kochert G, Yu Z H, et al. Molecular mapping of rice chromosomes. Theor Appl Genet, 1988, 76: 815829.
\[20\]Lincoln S E, Daly M J, Lander E S. Constructing genetics maps with Mapmaker/EXP3.0. Cambridge, MA: Whitehead Institute Technical Report, 1992.
\[21\]杨剑. 性状遗传分析方法研究及其软件开发\[学位论文\]. 杭州: 浙江大学, 2008.
\[22\]McCouch S R, Cho Y G, Yang M, et al. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 1113.
\[23\]陈静, 江玲, 王春明, 等.籼粳杂种花粉不育基因的定位. 作物学报， 2006, 32(4): 515521.
\[24\]Li W T, Zeng R Z, Zhang Z M, et al. Mapping of Sb locus for F1 pollen sterility in cultivated rice using PCR based markers. Acta Bot Sin, 2002, 44(4): 463467.
\[25\]Song X， Qiu S Q, Xu C G, et al. Genetic dissection of embryo sac fertility,pollen fertility, and their contributions to spikelet fertility of intersubspecific hybrids in rice. Theor Appl Genet, 2005, 110(2): 205211.
\[26\]严长杰, 梁国华, 顾名洪, 等. 典型籼粳杂种不育性的分子标记分析及其遗传基础. 遗传学报, 2003, 30(3): 267276.
\[27\]井文. 水稻籼粳亚种间杂种不育性的遗传分析\[学位论文\]. 南京: 南京农业大学， 2004.
\[28\]刘永胜, 朱立煌, 孙敬三, 等. 水稻籼粳杂种生殖障碍基因定位分析. 植物学报, 1997, 39(12): 10991104.
\[29\]Li Z K, Pinson S R M, Paterson A H, et al. Genetics of hybrid sterility and hybrid breakdown in an intersubspecific rice (Oryza sativa L.). Genetics, 1997, 145: 11391148.
\[30\]王春明, 安井秀, 万建民, 等. 水稻F2不育和抽穗期QTL分析. 遗传学报, 2002, 29(4): 339342.
\[31\]李晓玲. 水稻籼粳亚种间杂种不育性的研究进展. 中国农学通报, 2004, 20(5): 4549.
\[32］ Liu K D， Wang J， Li H B, et al. A genomewide analysis of wide compatibility in rice and the precise location of the S5 locus in the molecular map. Theor Appl Genet, 1997, 95: 809814.
\[33\]宋翔. 水稻籼、粳亚种间杂种不育机理的研究\[学位论文\]. 武汉: 华中农业大学， 2005.
