Genetic Diversity and Pathotype Variability of Xanthomans oryzae&nbsp; pv. oryzicola Strains

ZHANG Rongsheng1,2, CHEN Zhiyi1，*, LIU Yongfeng1

doi:10.3969/j.issn.10017216.2011.05.011

Chinese Journal OF Rice Science >

2011 , Vol. 25 >Issue 5: 523 - 528

DOI: https://doi.org/10.3969/j.issn.10017216.2011.05.011

Research Papers

Genetic Diversity and Pathotype Variability of Xanthomans oryzae pv. oryzicola Strains

Expand

¹ Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; ²College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;

Received date: 2010-10-14

Revised date: 2011-01-02

Online published: 2011-09-10

Fold

Abstract

Genetic diversity and pathotype variability of Xanthomans oryzae pv. oryzicola strains collected from Jiangsu, Yunnan, Jiangxi, Hunan and Anhui Provinces were analyzed by using repPCR fingerprinting techniques. Genomic DNAs from 69 strains were amplified with two specific primers BOX and ERIC. Dendrograms were generated from the data by using UPGMA analysis. The tested strains were classified into 10 and 8 groups at a similarity of 80% based on the amplification results with BOX and ERIC, respectively. The predominant groups were group 3 and Group 5 for the primers BOX and ERIC, involving 27(39.1%) and 24(348%) strains, respectively. The genetic diversities of the population of the tested strains were 0.9784 (with BOX) and 0.9833 (with ERIC), respectively, indicating the high genetic diversity of Xanthomans oryzae pv. oryzicola. According to inoculation results on five differential cultivars including nearisogenic rice lines carrying single resistance gene, IRBB4, IRBB5, IRBB14, IRBB21 and IRBB24, and a highly susceptible cultivar Jingang 30, the 69 strains were classified into 13 pathotypes. The predominant pathotype was C6 (26.1%). No relationships were observed between UPGMA groups (BOX and ERIC) of the 69 strains and pathotypes, but each group was partially associated with the regional origin of strains.

Key words： repPCR; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">︱Xanthomans oryzae ; pv. oryzicola; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">︱genetic diversity; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">︱pathotype

Cite this article

ZHANG Rongsheng1,2, CHEN Zhiyi1，*, LIU Yongfeng1 . Genetic Diversity and Pathotype Variability of Xanthomans oryzae pv. oryzicola Strains[J]. Chinese Journal OF Rice Science, 2011 , 25(5) : 523 -528 . DOI: 10.3969/j.issn.10017216.2011.05.011

References

\[1\]Ou S H. Rice Diseases. 2nd ed. Kew, England： Commonwealth Mycology Institute. 1985: 96103.

\[2\]Kriz R W. New, emerging, reemerging plant diseases in the United States. 1999. http://www.ces.ncsu.edu／depst. \[20100101\]

\[3\]Raymundo A K, Jr Briones A. Genetic diversity in Xanthomonas oryzae pv. oryzicola. IRRN, 1995, 20:35.

\[4\] Sheng Z J, Zhen L Y, Jun F X. Detection of QTL conferring resistance to bacterial leaf streak in rice chromosome 2 (O. sativa L. spp. indica). Sci Agric Sin,2005, 38： 19231925.

\[5\]姬广海, 许志刚, 张世珖. RepPCR技术对中国水稻条斑病菌的遗传多样性初析. 植物病理学报, 2002, 32(1): 2632.

\[6\]郑伟, 刘晓辉, 成国英, 等. 中国、日本和菲律宾水稻白叶枯病菌遗传多样性比较分析. 微生物学报, 2008, 25(4)： 519523.

\[7\]曾列先, 陈深, 张慧, 等. 广东水稻白叶枯病菌遗传多样性和小种分化研究. 植物病理学报, 2009, 39(3): 231237.

\[8\]姬广海, 许志刚. 水稻细菌性条斑病DNA多态性与致病性研究. 华中农业大学学报, 2000, 19(5): 430433.

\[9\]Gonzalez C, Szurek B, Manceau C, et al. Molecular and pathotypic characterization of new Xanthomonas oryzae strains from West Africa. Mol PlantMicrobe Interact, 2007, 20(5): 534546.

\[10\]郭亚辉, 许志刚, 胡白石, 等. 中国南方水稻条斑病菌小种分化研究. 中国水稻科学, 2004, 18(1):8385.

\[11\]姬广海, 何月秋, 张世光, 等. 水稻白叶枯病原菌遗传和致病型多样性分析. 华南农业大学学报, 2005, 26(2)： 2630.

\[12\]Adhikari T B, Mew T W, Leach J E. Genotypic and pathotypci diversity in Xanthomonas oryzae pv. oryzae in Nepal. Phytopathology, 1999, 89(7): 687694.

\[13\]Ochiai H, Horino O, Miyajima K, et al. Genetic diversity of Xanthomonas oryzae pv. oryzae strains from Sri Lanka. Phytopathology, 2000, 90(4): 415421.

\[14\]Pan R W, Zou W C, Xu D G, et al. Genotypic and pathotypic diversity of Xanthomonas oryzae pv. oryzicola in southern China. Phytopathology, 2010, 100(suppl 1): 96.

\[15\]梁传永, 李大春, 易建平, 等. 中国水稻条斑病毒力型的研究. 南京农业大学学报, 1994, 17(3)： 4852.

\[16\]Shekhawat G S, Srivastava D N. Epidemiology of bacterial leaf streak of rice. Ann Phytopath Soc Jpn, 1972, 38:734.

\[17\]徐羡明, 林壁润, 曾列先. 广东水稻细菌性条斑病菌致病力分化研究初报. 广东农业科学, 1992(6)： 3132.

\[18\]刘友勋, 成国英, 涂立超, 等. 中南4省水稻细菌性条斑病致病型比较. 华中农业大学学报, 2004, 23(5)： 504506.

\[19\]王绍雪, 马改转, 魏兰芳, 等. 西南地区水稻细菌性条斑病菌致病力的分化. 湖南农业大学学报, 2010, 36(2): 188191.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References