Mapping of QTLs for&nbsp; Flag Leaf Shape Using WholeGenome Resequenced Chromosome Segment Substitution Lines in Rice

XU Jianjun1,2, ZHAO Qiang3, ZHAO Yuanfeng1, ZHU Lei1, XU Chenwu1, GU Minghong1, HAN Bin3, LIANG Guohua1,*

doi:10.3969/j.issn.10017216.2011.05.005

Chinese Journal OF Rice Science >

2011 , Vol. 25 >Issue 5: 483 - 487

DOI: https://doi.org/10.3969/j.issn.10017216.2011.05.005

Research Papers

Mapping of QTLs for Flag Leaf Shape Using WholeGenome Resequenced Chromosome Segment Substitution Lines in Rice

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¹ Jiangsu Key Laboratory of Crop Genetics and Physiology /Key Laboratory of Plant Function Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; ² Songjiang Agricultural Technology and Popularization Center of Shanghai, Shanghai 201613, China; ³Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200233, China;

Received date: 2010-11-04

Revised date: 2011-01-03

Online published: 2011-09-10

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Abstract

Flag leaf shape is one of the most important target traits in rice ideal plant type breeding. Identification and localization of new rice flag leaf shape QTLs would be of great significance to facilitate the genetic analysis of complex traits, and provide QTLs/genes to develop new plant type rice varieties via markerassisted selection. One hundred and twenty eight chromosome segment substitution lines with 9311 as recipient and Nipponbare as donor were genotyped by wholegenome resequencing. The pairwise correlation analysis on three flag leaf shape traits was conducted. The correlations between flag leaf length and flag leaf area, and between flag leaf width and flag leaf area were significantly positive. Correlation analysis revealed that the flag leaf length and flag leaf area have a direct positive impact on spikelet number per panicle. By combining the sequencingbased Binmap with multiple linear regression analysis, four, four and six QTLs for flag leaf length, flag leaf width and flag leaf area were mapped, respectively, with contributions from 4.08% to 60.40%. The results provided a foundation for fine mapping and subsequent cloning of the QTLs.

Key words： rice; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">︱ flag leaf shape; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">︱ chromosome segment substitution line; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">︱ QTL mapping

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XU Jianjun1,2, ZHAO Qiang3, ZHAO Yuanfeng1, ZHU Lei1, XU Chenwu1, GU Minghong1, HAN Bin3, LIANG Guohua1,* . Mapping of QTLs for Flag Leaf Shape Using WholeGenome Resequenced Chromosome Segment Substitution Lines in Rice[J]. Chinese Journal OF Rice Science, 2011 , 25(5) : 483 -487 . DOI: 10.3969/j.issn.10017216.2011.05.005

References

\[1\]Delseny M, Salses J, Cooke R, et al. Rice genomices: Present and future. Plant Physiol Biochem, 2001, 39: 323334.

\[2\]左科生, 李育, 钟平安. 水稻理想株型与超高产育种的研究进展. 江西农业学报, 2003, 15(1): 3742.

\[3\]杨仁崔, 杨惠杰. 国际水稻研究所新株型稻研究进展. 杂交水稻, 1998, 13(5): 292231.

\[4\]黄耀祥, 林青山. 水稻超高产、特优质株型模式的构想与育种实践. 广东农业科学, 1994(4): 126.

\[5\]周开达, 马玉清, 刘太清. 穗重型杂交稻育种. 四川农业大学学报, 1995, 13(4): 403407.

\[6\]袁隆平. 杂交水稻超高产育种. 杂交水稻, 1997, 12(6): 16.

\[7\]方德义, 许传祯, 朱庆森, 等. 实用水稻栽培学. 上海: 上海科学技术出版社, 1981.

\[8\]Yoshidq S, Cook J H. Growth performance of an improved rice variety in the tropice. Int Rice Comm Newsl, 1971, 20: 115.

\[9\]丁颖. 丁颖稻作论文选集. 北京: 农业出版社, 1983.

\[10\]岳兵, 薛为亚, 罗利军, 等. 水稻剑叶部分形态生理特性QTL分析以及它们与产量、产量性状的关系. 遗传学报, 2006, 33(9): 824832.

\[11\]朱德峰. 水稻超高产途径与株型的研究\[学位论文\]. 南京: 南京农业大学, 2000.

\[12\]李仕贵, 何平, 王玉平, 等. 水稻剑叶性状的遗传分析和基因定位. 作物学报, 2000, 26(3): 261265.

\[13\]王一平, 曾建平, 郭龙彪, 等. 水稻顶部三叶与穗重的关系及其QTL分析. 中国水稻科学, 2005, 19(1): 1320.

\[14\]曹刚强, 高用明, 朱军. 多环境下水稻DH 群体剑叶长度的QTL分析. 作物学报, 2007, 33(2): 223229.

\[15\]童汉华, 梅捍卫, 邢永忠, 等. 水稻生育后期剑叶形态和生理特性的QTL 定位. 中国水稻科学, 2007, 21(5): 493499.

\[16\]彭茂民, 杨国华, 张菁晶, 等. 不同遗传背景下水稻剑叶形态性状的QTL 分析. 中国水稻科学, 2007, 21(3): 247252.

\[17\]肖珂, 左海龙, 巩迎军, 等. 控制水稻剑叶形态相关性状的数量基因位点(QTL)的定位. 上海师范大学学报：自然科学版, 2007, 36(2): 6670.

\[18\]王韵, 程立锐, 郑天清, 等. 影响水稻株高和剑叶宽主效QTL 对人工选择的响应. 中国水稻科学, 2009, 23(4): 363370.

\[19\]姜树坤, 张喜娟, 黄成, 等. 基于粳稻F2和F2:6群体的连锁图谱及剑叶性状QTL比较分析. 中国水稻科学, 2010, 24(4): 372378.

\[20\]Huang X H, Feng Q, Qian Q, et al. Highthroughput genotyping by wholegenome resequencing. Genome Res, 2009, 19(6): 10681076.

\[21\]Xu J J, Zhao Q, Du P N, et al. Developing high throughput genotyped chromosome segment substitution lines based on population wholegenome resequencing in rice(Oryza sativa L.). BMC Genomics, 2010, 11: 656.

\[22\]Paran I, Zamir D. Quantitative traits in plants: Beyond the QTL. Trends Genet, 2003, 19: 303306.

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