To compare the genetic diversity of rice landraces in different periods polymorphism, genetic similarity and cluster analyses were conducted with 148 rice landraces by using 48 SSR markers. The materials included 72 landraces collected during the 1970s (pastgrown landraces) and 76 grown during the past decade (currentgrown landraces) in Hani′s terraced fields in Yuanyang County, Yunnan Province. The results showed that the differences between the two periods in all indices, including average number of alleles, effective number of alleles, locus polymorphism information content and genotype diversity were not significant. Furthermore, 52 alleles of 24 SSR markers differed between the pastgrown and the currentgrown landraces, among which 32 alleles of 18 SSR markers were not detected in the currentgrown landraces and 20 alleles of 16 SSR markers were not detected in the pastgrown landraces. The average genetic similarity coefficient of the currentgrown landraces was 03340, and that of the pastgrown landraces was 03313. The currentgrown and pastgrown landraces were divided into japonica and indica subspecies at the genetic similarity coefficient of 0174 and 0203, respectively. These results indicated that for more than 30 years′ natural and artificial selection, there were variations in some of the allele locus of the rice landraces from Hani′s terraced fields with high genetic diversity. In addition, the indicajaponica subspecies differentiation was obvious in the landraces from Hani′s terraced fields in Yuanyang, and the differentiation seemed to be ongoing, indicating that Hani′s terraced fields and the rice landraces might be ideal tested field and materials for the study of indicajaponica subspecies evolution.
XU Fu-Rong1, 2 , DONG Chao1, YANG Wen-Yi1, TANG Cui-Feng1, A Xin-Xiang1, ZHANG En-Lai1, YANG Ya-Yun1, ZHANG Fei-Fei1, DAI Lu-Yuan1, * , ZHANG Hong-Sheng2, *
. Comparison of Genetic Diversity of Rice Landraces Planted in Two Periods in Hani′s Terraced Fields in Yuanyang County, Yunnan Province, China Using Microsatellite Markers[J]. Chinese Journal OF Rice Science, 2011
, 25(4)
: 381
-386
.
DOI: 10.3969/j.issn.1001-7216.2011.04.006
[1]应存山. 中国稻种资源. 北京: 中国农业科技出版社, 1993: 33.
[2]张启发. 绿色超级稻培育的设想. 分子植物育种, 2005, 3(5): 601-602.
[3]黎志康. 我国水稻分子育种计划的策略. 分子植物育种, 2005, 3(5): 602-608.
[4]程式华, 闵绍楷. 中国水稻品种: 现状与展望. 中国稻米, 2000(1): 13-16.
[5]郑康乐, 庄杰云. 我国水稻推广品种的遗传变异性和种质资源的开发利用. 中国农业科技导报, 2000, 2(3): 69-72.
[6]Zhu Y Y, Chen H R, Fan J H, et al. Genetic diversity and disease control in rice. Nature, 2000, 406: 718-722.
[7]Zhao W G, Chung J W, Ma K H, et al. Analysis of genetic diversity and population structure of rice cultivars from Korea, China and Japan using SSR markers. Gene Genom, 2009, 31(4): 283-292.
[8]徐福荣, 汤翠凤, 余腾琼, 等. 中国云南元阳哈尼梯田种植的稻作品种多样性. 生态学报, 2010, 30(12): 3346-3357.
[9]徐福荣, 张恩来, 董超, 等. 云南元阳哈尼梯田水稻地方品种表型多样性比较. 生物多样性, 2010, 18(4): 365-372.
[10]齐永文, 张冬玲, 张洪亮, 等. 中国水稻选育品种遗传多样性及其近50年变化趋势. 科学通报, 2006, 51: 693-699.
[11]Zhang H L, Sun J L, Wang M X, et al. Genetic structure and phylogeography of rice landraces in Yunnan, China, revealed by SSR. Genome, 2007, 50(1): 72-83.
[12]Thomson M J, Septiningsih E M, Suwardjo F, et al. Genetic diversity analysis of traditional and improved Indonesian rice (Oryza sativa L.) germplasm using microsatellite markers. Theor Appl Genet, 2007, 114: 559-568.
[13]魏兴华, 袁筱萍, 余汉勇, 等. 我国常规稻主栽品种的遗传变异分析. 中国水稻科学, 2009, 23(3): 237-244.
[14]Edwards K, Johnstone C, Thompson C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucl Acids Res, 1991, 19(6): 43-49.
[15]Botstein D, White R L, Skolnick M, et al. Construction of genetic linkage map in man using restriction fragment length polymorphisms. Am J Human Genet, 1980, 32: 314-331.
[16]Nei M. Analysis of gene diversity in subdivided population. Proc Natl Acad Sci USA, 1973, 70: 3231-3233.
[17]董玉琛, 曹永生, 张学勇, 等. 中国普通小麦初选核心种质的产生. 植物遗传资源学报, 2003, 4(1): 1-8.
[18]史军超. 中国湿地经典——红河哈尼梯田. 云南民族大学学报: 哲学社会科学版, 2004, 21(5): 77-81.
[19]角媛梅, 肖笃宁, 程国栋. 亚热带山地民族文化与自然环境和谐发展实证研究: 以云南省元阳县哈尼族梯田文化景观为例. 山地学报, 2002, 20(3): 266-271.
[20]姚敏, 崔保山. 哈尼梯田湿地生态系统的垂直特征. 生态学报, 2006, 26(7): 2115-2124.
[21]华蕾, 袁筱萍, 余汉勇, 等. 我国水稻主栽品种SSR多样性的比较分析. 中国水稻科学, 2007, 21(2): 150-154.
[22]程本义, 吴伟, 夏俊辉, 等. 利用微卫星标记分析浙江省近年来主要水稻品种的遗传多样性. 中国水稻科学, 2009, 23(5): 551-554.
[23]徐福荣, 董超，杨文毅, 等. 基于表型性状和SSR分子标记的云南省水稻主要育成品种（系）的遗传相似性. 植物遗传资源学报, 2011(印刷中).
[24]朱明雨, 王云月, 朱有勇, 等. 云南地方水稻品种遗传多样性分析及其保护意义. 华中农业大学学报, 2004, 23(2): 187-191.
[25]樊叶杨, 庄杰云, 吴建利, 等. 应用微卫星标记鉴别水稻籼粳亚种. 遗传, 2000, 22(6): 392-394.
[26]Yu S B, Xu W J, Vijayakumar C H M, et al. Molecular diversity and multilocus organization of the parental lines used in the international rice molecular breeding program. Theor Appl Genet, 2003, 108(1): 131-140.
[27]余显权, 蒋向辉, 吴海滨, 等. 贵州地方耐冷水稻品种的SSR遗传多样性分析. 西南农业学报, 2005, 18(1): 1-4.
[28]王一平, 魏兴华, 华蕾, 等. 不同地理来源旱稻种质资源的遗传多样性分析. 作物学报, 2007, 33(12): 2034-2040.
[29]角媛梅, 杨有洁, 胡文英, 等. 哈尼梯田景观空间格局与美学特征分析. 地理研究, 2006, 25(4): 624-632.
[30]胡文英, 角媛梅, 范弢. 哈尼梯田土地利用空间格局及其变化的信息图谱研究. 地理科学, 2008, 28(3): 419-424.
