Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2009 , Vol. 23 >Issue 6: 604 - 610

DOI: https://doi.org/10.3969/j.issn.1001-7216.2009.06.07

研究报告

Expression of Exogenous Xylanase Gene （atx） in Transgenic Rice

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  • 1College of Life Sciences, Zhejiang University, Hangzhou 310058, China; 2 Cixi Institute of Agricultural Sciences, Cixi 315300, China; 3 College of Animal Science, Zhejiang University, Hangzhou 310029, China; *Corresponding author, E-mail：xyweng@zju.edu.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2009-11-10

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Abstract

For improving the production efficiency of xylanase by expression of xylanase in transgenic rice plants, a xylanase gene (atx) was inserted into the binary expression vector pCAMBIA1301, and the resulting construction with atx was named atxRu3ep1301. The atxRu3ep1301 vector was introduced into rice (Oryza sativa L. subsp. japonica) variety Zhonghua 11 via Agrobacteriummediated transformation, by using the rice mature embryoderived callus as the explants. Both hygromycin resistance detection and PCR amplification confirmed that the target gene had been integrated into the genome of transgenic rice. RTPCR analysis of the total RNA extracted from the fresh leaves of several transgenic lines showed that the introduced xylanase gene could be normally expressed in rice under the control of CaMV 35S promoter. And in the leaves of transgenic rice, xylanase activity was up to 4.37 U/g in fresh leaf. In addition, growth and reproduction of the transgenic rice plants were not affected by the expression of foreign xylanase. Thus, it would be an economic way for xylanase production via transgenic rice.

Key words： rice; xylanase; construction of expression vector; transgene; bioreactor

Cite this article

HUANG Ying-ying,RONG Guo-zeng,HAN Ning,SUN Jian-yi,WENG Xiao-yan . Expression of Exogenous Xylanase Gene （atx） in Transgenic Rice
[J]. Chinese Journal OF Rice Science, 2009 , 23(6) : 604 -610 . DOI: 10.3969/j.issn.1001-7216.2009.06.07

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