Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2009 , Vol. 23 >Issue 5: 456 - 462

DOI: https://doi.org/10.3969/j.issn.10017216.2009.05.02

研究报告

Developmental Proteomics Analysis of Different Functional Leaves in Rice (Oryza sativa) at the Booting Stage

Expand
  • 1 Soil and Fertilizer & Resource and Environment Institute， Jiangxi Academy of Agricultural Sciences, Nanchang 330200， China; 2 Institute of AgroEcology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; *Corresponding author, E-mail：<A href=

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2009-09-10

Fold

Abstract

n order to reveal proteomic changes in rice functional leaves collected from four different leaf positions, the proteins expression profile of functional leaves at the booting stage in hybrid rice Shanyou 63 were studied by using the approach of plant proteomics. Protein expression profiles of the top leaves at the 13,14,15 and 16leaf stages(the total number of leaves of Shanyou 63 was 16) were investigated by twodimensional gel electrophoresis. Differential protein expression among the four stages were analyzed by Imagemaster 2D Elite 5.0, which allowed the identification of 23 significantly different gel spots. The spots were further verified by matrixassisted laser desorption/ionizationtime of flight mass spectrometry, in which 14 were identified. The functions of these 14 proteins were analyzed and the conclusion can be drawn from the following result: the major differential expression proteins (9 proteins) were associated with photosynthesis and respiration, three differential expression proteins were related to protein formation and protein function control, and the expression abundance of those 12 proteins increased regularly with the leaf position elevation; the other proteins(2 proteins) were involved in amino acid metabolism, showing an irregularly changing pattern in lower leaf positions, and a distinctly increasing expression abundance in flag leaf. The protein is the carrier of cell function and significantly influenced by the variation of cell function or intercellular environment, therefore, the reason that caused the above protein changes should be related to the development of leaves at different leaf positions.

Key words： rice; booting stage; leaf position; proteomics; differential expression proteome

Cite this article

SHAO Cai-hong ,XIE Jin-shui ,HUANG Yong-lan ,QIU Cai-fei,ZHANG Zhen-miao,LIN Wen-xiong . Developmental Proteomics Analysis of Different Functional Leaves in Rice (Oryza sativa) at the Booting Stage [J]. Chinese Journal OF Rice Science, 2009 , 23(5) : 456 -462 . DOI: 10.3969/j.issn.10017216.2009.05.02

References

[1]聂军, 郑圣先, 戴平安, 等.控释氮肥调控水稻光合功能和叶片衰老的生理基础.中国水稻科学, 2005, 19(3): 255-261.
[2]李德福, 李金才, 魏凤珍.拔节长穗期水分胁迫对旱作水稻若干生理特性和经济产量的影响.安徽农业科学, 2005, 33(7):1166-1167.
[3]王旭军, 徐庆国, 杨知建.水稻叶片衰老生理的研究进展.中国农学通报, 2005, 21(3): 187-191.
[4]周广生, 靳德明, 梅方竹. 水稻孕穗期干旱对籽粒性状的影响.华中农业大学学报, 2003, 22(3): 219-222.
[5]王琪, 王连敏.水稻孕穗期阶段性低温对结实及植株糖、氮含量的影响.吉林气象, 2000(4): 30-32.
[6]焦德茂, 李霞, 黄雪清, 等.不同高产水稻品种生育后期叶片光抑制、光氧化和早衰的关系.中国农业科学, 2002, 35(5): 487-492.
[7]王荣富, 张云华, 焦德茂, 等.超级杂交稻两优培九及其亲本生育后期的光抑制和早衰特性.作物学报, 2004, 30(4): 393-397.
[8]金松恒, 蒋德安, 王品美, 等.水稻孕穗期不同叶位叶片的气体交换与叶绿素荧光特性. 中国水稻科学, 2004, 18(5): 443-448.
[9]孙骏威, 黄莹莹, 徐坤, 等.低钾对钾迟钝型水稻不同叶位叶片光合活性的影响. 浙江农业学报, 2006, 18(5)： 354-358.
[10] Cui S X, Huang F, Wang J, et al. A proteomic analysis of cold stress responses in rice seedlings. Proteomics, 2005, 5: 3162-3172.
[11]Zhang Y P, Jin F, Chai X Q, et al. 2DE method and proteomic map analysis in rice leaves under iron deficient stress. China J Bioinform, 2004, 2(3): 6-10.
[12]Antonius K, Michael P, Washbum B, et al. Proteomic survey of metabolic pathways in rice. Proc Natl Acad Sci USA, 2002, 99(18): 11969-11974.
[13]Trisiriro J A, Jeyachok N, Chen S T. Proteomics characterization of different bran proteins between aromatic and nonaromatic rice(Oryza sativa L. ssp. indica). Proteomics, 2004, 4: 2047-2057.
[14]Hajduch M, Rakwal R, Garawal G K, et al. Highresolution twodimensional electrophoresis separation of proteins from metalstressed rice(Oryza sativa L.) leaves: Drastic reductions/fragmentation of ribulose1,5-bisphosphate carboxylase/oxygenase and induction of stress-related proteins. Electrophoresis, 2001, 22(13): 2824-2831.
[15]Nazrul I, Lonsdale M, Upadhyaya N M, et al. Protein extraction from mature rice leave for twodimensional gel electrophoresis and its application in proteome analysis. Proteomics, 2004, 4: 1903-1908.
[16]毛达如.植物营养研究方法.北京: 中国农业大学出版社，2000: 16.
[17]Wang X C, Li X F, Li Y X. A modified coomassie brilliant staining method at nanogram sensitivity compatible with proteomic analysis. Biotech Lett, 2007, 29: 1599-1603.
[18] 夏其昌, 曾嵘. 蛋白质化学与蛋白质组学. 北京: 科学出版社, 2004: 278.
[19]Kimural N. Nucleoside diphosphate kinases: Genes and protein functions. J Bioenerg Biomembr, 2003, 35(1): 3-4.
[20]Hasunuma K, Yabe N, Yoshida Y, et al. Putative functions of nucleoside diphosphate kinase in plants and fungi. J Bioenerg Biomembr, 2003, 35(1): 57-65.
[21] Lascul L, Gonin P. The catalytic mechanism of nucleoside diphosphate kinases. J Bioenerg Biomembr, 2000, 32(3): 237-246.
[22]Leung K C, Li H Y, Mishra G, et al. ACBP4 and ACBP5, novel Arabidopsis acylCoAbinding proteins with kelch motifs that bind oleoylCoA. Plant Mol Biol, 2004, 55: 297-309.
[23]Thomas G， Luther H. Transcriptional and translational control of cytoplasmic proteins after serum stimulation of quiescent Swiss 3T3 cells. Proc Natl Acad Sci USA, 1981, 78(9): 5712-5716.
[24]Yenofsky R， Bergmann I， Brawerman G. Messenger RNA species partially in a repressed state in mouse sarcoma ascites cells. Proc Natl Acad Sci USA, 1982, 79(19): 5876-5880.
[25]李颖, 冯建生. 真核生物mRNA的帽结构与帽结合蛋白. 生命科学研究, 1999, 3(4): 286-291.
[26] 韩飞, 王高. 真核生物mRNA稳定性的控制机制. 安徽农学通报, 2007, 13(11): 27-29.
[27] 逯斌, 谭克辉. 冬小麦春化过程中低温诱导的与花芽分化相关的mRNA和蛋白质的合成. 植物生理学报, 1992, 18(2): 113-120.
[28] 张经余, 赵志虎, 蔡民华. GroEL分子伴侣研究进展. 生物技术通讯, 2001, 12(2): 127-129.
[29] 邹承鲁. 第二遗传密码——新生肽链及蛋白质折叠的研究. 长沙: 湖南科学技术出版社, 1997: 173-175.
Options
Outlines

/

〈 〉

Tel: 0571-63370278　E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.