<FONT face="Verdana">Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice </FONT>

MA Bingtian,﹟,WAN Jia,﹟,ZHANG Qi,﹟,JIANG Bin,XU Yongju,LI Dongmei,XU Zhengjun

doi:10.3969/j.issn.1001-7216.2009.04.02

Chinese Journal OF Rice Science >

2009 , Vol. 23 >Issue 4: 342 - 348

DOI: https://doi.org/10.3969/j.issn.1001-7216.2009.04.02

研究报告

Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice

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¹Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; ²Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China; ³ Plant Quarantine Station of Sichuan Agriculture Department, Chengdu 610041, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: mywildrice@yahoo.com.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2009-07-10

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Abstract

OsHSP90 is an abiotic stressinducible gene in rice. From the chilling and droughtinducible cDNA library of rice, a novel gene, OsHSPBP, which interacted with OsHSP90 was identified by using yeast twohybrid system. The 1321 bp full length cDNA of OsHSPBP was cloned. Its open reading frame (ORF) predicts a 244amino acid(aa) polypeptide with calculated molecular weight of 25.5 kD. A tify motif and a CCT_2 motif were found at the aa 97-132 and aa 185-211, respectively. Sequence similarity analysis indicated that the OsHSPBP had 30.66%-70.40% identities with the tify family proteins from other plants. Among the putative promoter region, six factors matched to ciselements related to the abiotic stress response were identified using the PlantCARE software. Semiquantitative RTPCR showed that the OsHSP90 and OsHSPBP might be detected in the similar degree under different stresstreatments, suggesting that OsHSP90 and OsHSPBP could be involved in the signal transmission and response to the abiotic stress in rice.

Key words： rice; abiotic stress; inducible gene; gene expression; amino acid sequence; yeast twohybrid system

Cite this article

MA Bingtian,﹟,WAN Jia,﹟,ZHANG Qi,﹟,JIANG Bin,XU Yongju,LI Dongmei,XU Zhengjun . Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice [J]. Chinese Journal OF Rice Science, 2009 , 23(4) : 342 -348 . DOI: 10.3969/j.issn.1001-7216.2009.04.02

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