Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2011 , Vol. 25 >Issue 3: 261 - 266

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.03.006

研究报告

Genetic Analysis and Molecular Mapping of a Rice Rumpled and Twisted Leaf Mutant (rtl1)

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  • 1State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 2College of Life and Environment Science, Hangzhou Normal University, Hangzhou 310016, China; 3Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; *Corresponding author, E-mail: qianqian188@hotmail.com

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2011-05-10

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Abstract

A rumpled and twisted leaf 1 (rtl1) mutant was derived from japonica cultivar Nipponbare by ethyl methane sulfonate (EMS) treatment, which was characterized by rumpled and twisted leaf at the seedling stage. The F2 population was constructed by crossing with indica cultivars TN1 and Zhefu 802, respectively. Genetic analysis confirmed that it was controlled by one recessive nuclear gene. The closely linked SSR marker RM1155 was obtained through bulked segregant analysis．Subsequently, new STS markers were developed using published rice genome sequence, and the gene was finally located between the STS marker T1591 and SSR marker RM1359 with the distances of 0.48 and 0.96 cM, respectively. This will contribute to cloning of the target gene in further studies.

Key words： rice; leaf type; genetic analysis; gene mapping; molecular marker

Cite this article

FANG Yun-xia,SONG Xiu-juan,PENG You-lin,DONG Guo-jun,GUO Long-biao,ZENG Da-li,ZHANG Guang-heng,YAN Hong-lan,QIAN Qian . Genetic Analysis and Molecular Mapping of a Rice Rumpled and Twisted Leaf Mutant (rtl1)[J]. Chinese Journal OF Rice Science, 2011 , 25(3) : 261 -266 . DOI: 10.3969/j.issn.1001-7216.2011.03.006

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