<FONT face="Verdana">Analysis of Special DNA Methylated Sites Between Haploid  of TwinSeedling and Its Hybrids in Rice</FONT>

WU Shao-hua,XUE Jing-jing,ZHANG Hong-yu,XU Pei-zhou,WU Xian-jun*

doi:10.3969/j.issn.1001-7216.2011.03.004

Chinese Journal OF Rice Science >

2011 , Vol. 25 >Issue 3: 249 - 255

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.03.004

研究报告

Analysis of Special DNA Methylated Sites Between Haploid of TwinSeedling and Its Hybrids in Rice

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Rice Research Institute/Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu 611130, China; *Corresponding author, E-mail: wuxjsau@126.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2011-05-10

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Abstract

The DNA cytosine methylation mutation at the 5′CpCpGpG sites of the haploid of SARII628, and its hybrids with Shuhui 527, Shuhui 363 were analyzed by employing the MSAP (methylation sensitive amplification polyphisim) method. A total of 765 DNA methylated sites were detected and the methylation level of hybrids was lower than that of the parents. Meanwhile, different bands between hybrids and parents were analyzed and two types of methylated sites were detected, of which one inherited from the haploid, and the other didn′t. The biological functions of genes related to methylated sites involved in cell structure, metabolize and stress reaction. Therefore, DNA methylated modifications may be involved in the regulation of related genes, playing an important role in plant growth, development and envolution.

Key words： twin-seedling rice; haploid; methylation sensitive amplification polymorphism; DNA methylation

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WU Shao-hua,XUE Jing-jing,ZHANG Hong-yu,XU Pei-zhou,WU Xian-jun* . Analysis of Special DNA Methylated Sites Between Haploid of TwinSeedling and Its Hybrids in Rice[J]. Chinese Journal OF Rice Science, 2011 , 25(3) : 249 -255 . DOI: 10.3969/j.issn.1001-7216.2011.03.004

References

[1]吴乃虎. 基因工程原理. 北京: 科学出版社, 2001: 86-88.

[2]朱玉贤, 李毅. 现代分子生物学. 北京: 高等教育出版社, 2002: 250-254.

[3]Richards E J. DNA methylation and plant development. Trends Genet, 1997, 13: 319-323.

[4]Chan S W, Henderson I R, Jacobsen S E. Gardening the genome: DNA methylation in Arabidopsis thaliana. Nat Rev Genet, 2005, 6(5): 351-360.

[5]Bird A P, Wolffe A P. Methylation-induced repression： Belts, braces, and chromatin. Cell, 1999, 99（5）: 451-454.

[6]Bestor T H. The DNA methyltransferases of mammals. Human Mol Genet, 2000, 9(16): 2395-2402.

[7]Bender J. DNA methylation and epigenetics. Annu Rev Plant Biol, 2004, 55: 41-68.

[8]Gehring M, Choi Y, Fischer R L. Imprinting and see development. Plant Cell, 2004, 16(Suppl): S203-S213.

[9]Wassenegger M. RNA-directed DNA methylation. Plant Mol Biol, 2002, 43: 203-220.

[10]Masterson J. Stomatal size in fossil plants evidence for polyploidy in majority of angiosperms. Science, 1994, 264: 421-424.

[11]Bowers J E, Chapman B A, Rong J, et al. Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events. Nature, 2003, 422: 433-438.

[12]Zhang H Y, Peng H, LI Y, et al. Patterns of DNA cytosine methylation between haploids and corresponding diploids in rice. Chin Sci Bull, 2006, 51(14): 1721-1728.

[13]Peng H, Zhang H Y, Li Y, et al. Natural homologous triploidization and DNA methylation in SARII-628, a twin-seedling rice (Oryza sativa L.). Rice Sci, 2007, 14(4): 265-271.

[14]Xiong L Z, Xu C G, Saghai Maroof M A, et al. Patterns of cytosine methylation in an elite rice hybrid and its parental lines detected by a methylation-sensitive amplification polymorphism technique. Mol Gen Genet, 1999, 261: 439-446.

[15]Mcclelland M, Nelson M, Raschke E. Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucl Acids Res, 1994, 22(17): 3640-3659.

[16]Reyna-Lopez G E, Simpson J, Ruiz-Herrera J. Differences in DNA methylation patterns are detectable during the dimorphic transition of fungi by amplification of restriction polymorphism. Mol Gen Genet, 1997, 253(6): 703-710.

[17]Okuda K, Nakamura T, Sugita M, et al. A pentatricopeptide repeat protein is a site recognition factor in chloroplast RNA editing. JBC, 2006, 281(49): 37661-37667.

[18]Zehrmann A, Verbitskiy D, van der Merwe J A, et al. A DYW domain-containing pentatricopeptide repeat protein is required for RNA editing at multiple sites in mitochondria of Arabidopsis thaliana. Plant Cell, 2009, 21: 558-567.

[19]Yi Z B, Sun Y, Niu T T, et al. Patterns of DNA cytosine methylation between hybrids and their parents in sorghum genome. Acta Agron Sin, 2005, 31(9): 1138-1143.

[20]朱新霞, 汪保华, 郭旺珍, 等. 中棉所12配制的2个杂交棉DNA甲基化遗传与传递. 作物学报, 2009, 35(12): 2150-2158.

[21]He G M, Zhu X P, Elling A A, et al. Global epigenetic and transcriptional trends among two rice subspecies and their reciprocal hybrids. Plant Cell, 2010, 22: 17-33.

[22]吴先军, 汪旭东, 周开达, 等. 从农艺性状和分子标记鉴定一个水稻三倍体×二倍体 F2 群体的遗传稳定性. 植物学报, 1999, 41(10): 1067-1071.

[23]李云, 张红宇, 汪秀志, 等. 水稻同源三倍体（149-B）×二倍体F2代稳定群体的验证及分析. 遗传学报, 2004, 31(6): 604-608.

[24]Feng S, Cokus S J, Zhang X, et al. Conservation and divergence of methylation patterning in plants and animals. Proc Natl Acad Sci USA, 2010, 107(19): 8689-8694.

[25]Zemach A, McDaniel I E, Silva P, et al. Genome-wide evolutionary analysis of eukaryotic DNA methylation. Science, 2010, 328(5980): 916-919.

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