Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2011 , Vol. 25 >Issue 1: 31 - 36

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.01.005

研究报告

Efficient Selection of Fine Rice Quality Maintainer Lines with Functional Markers of Wx and fgr Genes

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  • 1China National Rice Research Institute, Hangzhou 310006, China; 2College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; *Corresponding author, E-mail: <A href=

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2011-01-10

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Abstract

According to the genotypes detected by two primers of functional markers, 484/485 (for Wx gene) and fgr1F/1R(for fgr gene), amylose contents and fragrance of 13 indica maintainer lines, Yixiang 1B was selected as good rice quality donor parent. The two markers were used to genotype the F2 individual plants derived from Ⅱ32B/Yixiang 1B. There were significant difference in electrophoretogram among individual plants, so that they were easy to distinguish, and these two markers showed independent inheritance. Superior individuals with fgr and Wx genotypeⅠ(same as Yixiang 1B) and Ⅲ (heterozygote) were selected. Then combined with the selection of agronomic and rice quality characters, the detection in selected plants or individual plants in F3 to F6 generations were carried out. In F6 generation, the plants were detected by 484/485 and fgr1F/1R again, advanced plants were obtained, which had stable characters, good plant type, obvious fragrance, medium amylose content and so on. Results of testcross showed that 75.5% plants were maintainer lines. It is suggested that detection and selection of maintainer lines of indica rice with functional markers of Wx and fgr genes in earlygeneration combined with conventional breeding could provide a rapid way to obtain good rice quality maintainer lines with fragrance, medium amylose content, slender grain shape, low chalkiness degree, high transparency and so on.

Key words： indica rice; markerassisted selection; amylose content; fragrance; functional marker

Cite this article

CHEN Shen-guang,ZHOU Yi-feng,ZHAO Fei,JIN Liang,SHEN Sheng-quan . Efficient Selection of Fine Rice Quality Maintainer Lines with Functional Markers of Wx and fgr Genes [J]. Chinese Journal OF Rice Science, 2011 , 25(1) : 31 -36 . DOI: 10.3969/j.issn.1001-7216.2011.01.005

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