<FONT face="Verdana">A Simple Method for Rapid Preparation of Rice Genomic DNA</FONT>

SUN Chuan,CHEN Gang,RAO Yu-chun,ZHANG Guang-heng,GAO Zhen-yu,LIU Jian,JU Pei-na,HU Jiang,GUO Long-biao,QIAN Qian,ZENG Da-li

doi:10.3969/j.issn.1001-7216.2010.06.021

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 6: 677 - 680

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.06.021

实验技术

A Simple Method for Rapid Preparation of Rice Genomic DNA

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¹State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; ²Agricultural College, Yangzhou University, Yangzhou 225009, China; ^#These authors contributed equally to this paper; *Corresponding authors, E-mail: qianqian188@hotmail.com; dalizeng@126.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-11-10

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Abstract

A simple method for preparation of rice genomic DNA was developed. A small amount (1- 50 mg) of leaf tissue of rice seedling, 500 μL of extraction buffer, and one steel bead were put into a 2mL microcentrifuge tube. After vigorously mashing for 2 min, 5 μL of the supernatant was directly applied to PCR amplification. Otherwise, the supernatant was precipitated with 2 volumes of ethanol to obtain high quality genomic DNA. This method is simple, rapid, low cost, and reliable for PCR analysis. One person can manipulate as many as 96 samples for PCR in 10 minutes. It is especially suitable for genotyping of large number of samples.

Key words： DNA extraction; high-throughput PCR amplification; sample preparation; methodology; rice

Cite this article

SUN Chuan,CHEN Gang,RAO Yu-chun,ZHANG Guang-heng,GAO Zhen-yu,LIU Jian,JU Pei-na,HU Jiang,GUO Long-biao,QIAN Qian,ZENG Da-li . A Simple Method for Rapid Preparation of Rice Genomic DNA[J]. Chinese Journal OF Rice Science, 2010 , 24(6) : 677 -680 . DOI: 10.3969/j.issn.1001-7216.2010.06.021

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