Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 6: 652 - 654

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.06.015

研究简报

Gnetic Analysis and Gene Mapping of a Short Root Mutant ksr1 in Rice

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  • Laboratory of Plant Molecular Biology, Ningbo University, Ningbo 315211, China; *Corresponding author, E-mail: zhushihua@nbu.edu.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-11-10

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Abstract

A short root mutant ksr1 with the Kasalath background was isolated from an EMSmutagenized population in rice. The root length of 6dayold ksr1 plants was only about 20% of the wild type. Genetic analysis indicated that the short root phenotype of ksr1 was controlled by a recessive mutation in a single nuclearencoded gene. To map the ksr1 mutation, an F2 population was generated by crossing the ksr1 mutant with Nipponbare. The KSR1 locus was linked to the SSR marker RM1223 on chromosome 4. Another eight SSR markers and two InDel markers were developed around this marker. The KSR1 gene was further mapped to a 155 kb region, flanked by the InDel marker 424725K and the SSR marker RM17182.

Key words： rice; short root; gene mapping; molecular marker

Cite this article

NING Yong-qiang,DING Wo-na,ZHU Shi-hua*,YU Hong-wei,YU Hong,LU Kai-xing . Gnetic Analysis and Gene Mapping of a Short Root Mutant ksr1 in Rice[J]. Chinese Journal OF Rice Science, 2010 , 24(6) : 652 -654 . DOI: 10.3969/j.issn.1001-7216.2010.06.015

References

[1]Vernoux T, Wilson R C, Seeley K A, et al. The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development. Plant Cell, 2000, 12: 97-109.
[2]Blilou I, Frugier F, Folmer S, et al. The Arabidopsis HOBBIT gene encodes a CDC27 homolog that links the plant cell cycle to progression of cell differentiation. Genes & Dev, 2002, 16: 2566-2575.
[3]Cano-Delgado A, Metzlaff K, Bevan M W. The eli1 mutation reveals a link between cell expansion and secondary cell wall formation in Arabidopsis thaliana. Development, 2000, 127: 3395-3405.
[4]Fagard M, Desnos T, Desprez T, et al. PROCUSTE1 encodes a cellulose synthase required for normal cell elongation specifically in roots and dark-grown hypocotyls of Arabidopsis. Plant Cell, 2000, 12: 2409-2424.
[5]Jia L Q, Zhang B T, Mao C Z, et al. OsCYT-INV1 for alkaline/neutral invertase is involved in root cell development and reproductivity in rice (Oryza sativa L.). Planta, 2008, 228: 51-59.
[6]Jiang H W, Wang S M, Dang L, et al. A novel short-root gene encodes a glucosamine-6-phosphate acetyltransferase required for maintaining normal root cell shape in rice. Plant Physiol, 2005, 138: 232-242.
[7]Li J, Zhu S H, Song X W, et al. A rice glutamate receptor like gene is critical for the division and survival of individual cells in the root apical meristem. Plant Cell, 2006, 18: 340-349.
[8]Yoshida S, Forno D A , Cock J H, et al. Laboratory Manual for Physiological Studies of Rice. 3rd ed. Manila: IRRI, 1976: 61-64.
[9]吴伟明，程式华. 水稻根系育种的意义与前景. 中国水稻科学, 2005, 19(2): 174-180.
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