Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 5: 551 - 558

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.05.019

综述与专论

Progress on the Study of the Bentazon Sensitive Mutants in Rice

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  • College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; E-mail: zhjwen@zju.edu.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-09-10

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Abstract

Two rice bentazonlethal mutants, Norin 8m and M8077S, were obtained from japonica and indica rice independently by γray radiation in Japan and China. Both mutants were sensitive to the sulfonylurea herbicides, but insensitive to the other hebicides. Genetic analysis showed that both mutants were controlled by a single recessive gene, and the sensitivities to bentazon and sulfonylurea were controlled by the same gene. Gene mapping, gene cloning and complementation experiment showed that both Norin 8m and M8077S were resulted from a singlebase deletion of CYP81A6, a cytochrome P450 gene. A singlebase deletion of C at the 507th nucleotide coding sequence starting from ATG of CYP81A6 in Norin 8m were identified, compared with a singlebase deletion of G at the 2058th nucleotide in coding sequence starting from ATG of CYP81A6 in M8077S. The bentazonlethal mutants have been used for hybrid seed reproduction to ensure hybrid seed purity for the twoline hybrid seed production. Three photothermosensitive genic male sterility (P/TGMS) lines (M8064S, B88S, and B63S) marked with bentazonlethality and 3 relevant hybrids (Fengliangyou 2, Benliangyou 639, and Benliangyou 9) have been successfully developed and registered in Hubei and Jiangxi Provinces, China. These bentazonlethal mutants have been also used for mixedplanting hybrid seed production. A bentazonsenstive restorer line MC526 and its matched hybrid combination Hunzhi 1 have been developed successfully. The procedures of mixed planting and the key techniques for mechanized production of hybrid seeds have been set up. The paper also reviewed the future applications of CYP81A6 gene in herbicide resistant rice breeding, the safety control of genetically modified rice and the application prospects of chemical emasculation.

Key words： mutant; bentazon; sulfonylurea; hybrid rice; herbicide resistance

Cite this article

ZHANG Ji-wen . Progress on the Study of the Bentazon Sensitive Mutants in Rice [J]. Chinese Journal OF Rice Science, 2010 , 24(5) : 551 -558 . DOI: 10.3969/j.issn.1001-7216.2010.05.019

References

[1]Mori K. Inheritance of susceptible mutant in rice plant to herbicide bentazon. Jpn J Breeding, 1984, 34(Suppl 1): 421-422.
[2]张集文, 武晓智. 水稻光-温敏雄性不育系化学致死突变体的诱变筛选与初步研究. 中国水稻科学, 1999, 13(2): 65-68.
[3]向太和, 杨剑波, 黄大年. 水稻突变体对除草剂苯达松敏感致死的机理研究. 农药, 2004, 43(5): 217-220, 232.
[4]Zhang J W, Xu Y B, Wu X Z, et al. Bentazon and sulfonyl-urea sensitive mutant: Breeding, genetics and potential application in seed production of hybrid rice. Theor Appl Genet, 2002, 105: 16-22.
[5]Pan G, Zhang X Y, Liu K D, et al. Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides. Plant Mol Biol, 2006, 61: 933-943.
[6]苏少泉, 宋顺祖. 中国农田杂草化学防治. 北京: 中国农业出版社, 1996: 170-176.
[7]Retzlaff G, Hamm R. The relationship between CO2 assimilation and the metabolism of bentazone in wheat plants. Weed Res, 1976, 16: 263-266.
[8]Mine A, Miyakade M, Matsunaka S. The mechanism of bentazon selectivity. Pestic Biochem Physiol, 1975, 5(6): 566-574.
[9]McFadden J J, Gronwald J W, Eberlein C V. In vitro hydroxylation of bentazon by microsomes from naphthalic anhydride-treated corn shoots. Biochem Biophys Res Commun, 1990, 168: 206-213.
[10]Burton J D, Maness E P. Constitutive and inducible bentazon hydroxylation in shatter cane (Sorghum bicolor)and Johnson grass (S.halapense). Pestic Biochem Physiol, 1992, 44(1): 40-49.
[11]Brown H M. Mode of action, crop selectivity and soil relations of the sulfonylurea herbicides. Pestic Sci, 1990, 29(3): 263-281.
[12]郑明奇, 邱立红, 张文吉. 细胞色素P450s酶系催化除草剂代谢的研究进展. 农药学学报, 2004, 5(3): 1-8.
[13]Wang Y S, Lu X Z, Sun M N, et al. Physiological research on the difference of bentazon tolerance in wild type rice and sensitive lethal mutants. Acta Agron Sin, 2008, 34(9): 1615-1622.
[14]王云生, 陆徐忠, 王智勇, 等. 适用于水稻微粒体蛋白的双向电泳技术及苯达松抗性相关蛋白的初步分析. 作物学报, 2007, 33(8): 1340-1345.
[15]朱剑. 水稻Bel基因的克隆、表达特性分析及对烟草的转化[D]. 南昌: 南昌大学, 2007.
[16]张集文, 武晓智. 苯达松致死标记两用不育系8077S 选育及其应用. 杂交水稻, 2000, 15(6): 5-8.
[17]朱必凤, 朱友林, 吴成钢, 等. 对除草剂敏感致死水稻bel 基因的RAPD 和SCAR 分子标记. 作物学报， 2006, 32(4): 618-624.
[18]杨剑波, 向太和, 李莉, 等. 水稻苯达松敏感致死基因（bel）的电子杂交定位和基因预测. 作物学报, 2004, 30(11): 1152-1158.
[19]刘秋华, 陆作楣. 水稻农林8号m苯达松敏感致死基因的初步定位. 南京农业大学学报, 2004, 27(4): 17-19.
[20]朱磊, 朱友林, 余潮, 等. 水稻苯达松敏感致死基因bel的初步定位. 南昌大学学报: 理科版, 2005, 29(6): 540-542.
[21]朱磊. 稻苯达松敏感致死基因bel的精细定位[D]. 南昌: 南昌大学, 2005, 6.
[22]李飞. 水稻bel基因区域的单核苷酸多态性及bel基因的精细定位[D]. 南昌: 南昌大学, 2006.
[23]Zhang L, Lu Q, Chen H G, et al. Identification of a cytochrome P450 hydroxylase, CYP81A6, as the candidate for the bentazon and sulfonylurea herbicide resistance gene, Bel, in rice. Mol Breeding, 2007, 19:59-68.
[24]Tu J M, Zhang J W, Pan G, et al. A bendazon and sulfonylurea herbicide-resistant gene CYP81A6 of rice: International Application No.: PCT/CN2005/000936, Publication No.:WO/2007/000077[P]. 2007-04-01.
[25]Werck-Reichhart D, Bak S, Paquette S. Cytochromes P450//Somerville C R, Meyerowitz E M. The Arabidopsis Book. MD: American Society of Plant Biologists, 2002: 1-28.
[26]张集文, 武晓智. 一种从水稻杂交种中除去母本自交种苗的技术: 中国, CN1217870[P]. 1999-06-02.
[27]张集文, 武晓智. 植物化学致死标记及其在作物杂交优势利用中的应用. 湖北农业科学, 1999(4): 27-29.
[28]周桂香, 黄作成, 陈祥付. 丰两优二号杂交水稻高产栽培技术要点. 种子世界, 2007(4): 47.
[29]胡旭. 基因标记纯度安全两系杂交水稻新品种苯两优9 号. 作物杂志, 2006(3): 63.
[30]三井东压化学株式会社. 一种培育杂交水稻种子的方法： 中国, CN1036120[P]. 1989-10-11.
[31]Maruyama K, Kato H. Araki H. Mechanized production of F1 seeds in rice by mixed planting. Japan Agric Res Quart, 1991, 4: 243-252.
[32]朱启升, 王安东, 杨前进, 等. 水稻除草剂敏感基因导入恢复系的研究.杂交水稻, 2000, 15(3): 5-6.
[33]朱启升. 杂交水稻混播制种技术研究进展. 作物研究, 2004, 4: 204-207.
[34]Wang S M, Zhu Q S, Wang W L, et al. The application of bentazon susceptible gene on seed production of hybrid rice. Agri Sci ＆ Technol, 2008, 9(1): 99-103, 145 .
[35]张德文, 陈多璞, 杨前进, 等. 不同时期喷施苯达松对混制1号制种产量的影响. 安徽农业科学, 2007, 35(11): 3212-3213, 3215.
[36]张德文, 杨前进, 王士梅, 等. 混制1号机械化混播制种生产技术. 中国农学通报, 2008, 24(10): 66-69.
[37]张集文, 武晓智. 一种杂交水稻种子生产技术： 中国， CN1429478[P]. 2003-07-16.
[38] Lin C Y, Fang J, Xu X L, et al. A built-in strategy for containment of transgenic plants: Creation of selectively terminable transgenic rice. PLoS One, 2008, 3(3): e1818.
[39]张集文, 涂巨民. 培育化学去雄和无籽果实植物的方法： 中国, CN1664101[P]. 2005-09-07.
[40]欧阳天赞, 谢九皋, 李学垣. 磺酰脲类除草剂在土壤中的吸附与降解研究进展. 华中农业大学学报, 2004, 23(3): 375-379.
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