<FONT face="Verdana">Interaction Between  Rice Stripe Virus   NS3 Protein and  Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) of Rice </FONT>

XIAO  Dong-lai,JIA  Dong-sheng,WU  Jian-guo,DU  Zhen-guo,XIE  Li-yan,WU  Zu-jian*,XIE  Lian-hui*

doi:10.3969/j.issn.1001-7216.2010.05.008

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 5: 493 - 496

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.05.008

研究报告

Interaction Between Rice Stripe Virus NS3 Protein and Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) of Rice

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Key Laboratory of Plant Virology of Fujian Province/Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; *Corresponding author, E-mail: wuzujian@126.com; fjxlh@126.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-09-10

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Abstract

NS3 protein of rice stripe virus (RSV) is an RNA silencing suppressor. Yeast twohybrid assay was used to screen the rice cDNA library with the NS3 protein as a bait. A novel rice protein interacting with RSV NS3 was obtained. The putative function of the interacting protein was glyceraldehyde3phosphate dehydrogenase (GAPDH). Bimolecular fluorescence complementation (BiFC) also showed that RSV NS3 could interact with the GAPDH. Cellular localization studies showed that GAPDHGFP fusion protein accumulated predominantly in cytoplasm of tobacco (Nicotiana benthamiana) through transient expression assay. Possible functions of GAPDH during the infection of rice by RSV were discussed.

Key words： rice stripe virus; protein function; interaction; yeast two hybrid; bimolecular fluorescence complementation

Cite this article

XIAO Dong-lai,JIA Dong-sheng,WU Jian-guo,DU Zhen-guo,XIE Li-yan,WU Zu-jian*,XIE Lian-hui* . Interaction Between Rice Stripe Virus NS3 Protein and Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) of Rice [J]. Chinese Journal OF Rice Science, 2010 , 24(5) : 493 -496 . DOI: 10.3969/j.issn.1001-7216.2010.05.008

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